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Catalog Number	Size	Price
LS-C159363-400	400 µl	$393

MAP1LC3A / LC3A Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

MAP1LC3A / LC3A Antibody - 5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraformaldehyde. Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.

MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

MAP1LC3A / LC3A Antibody - Western blot of anti-cleaved-LC3 (APG8a) antibody in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified antibody.

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Polyclonal Rabbit anti‑Human MAP1LC3A / LC3A Antibody (aa89‑120, IHC, IF, WB) LS‑C159363

Name: Anti-MAP1LC3A / LC3A Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C159363-400
Price: 393.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

MAP1LC3A / LC3A Rabbit anti-Human Polyclonal (aa89-120) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$393

LS-C159363-400

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MAP1LC3A / LC3A Rabbit anti-Human Polyclonal (aa89-120) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

LC3A antibody LS-C159363 is an unconjugated rabbit polyclonal antibody to LC3A (MAP1LC3A) (aa89-120) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human MAP1LC3A / LC3A

Synonyms

See All MAP1LC3A / LC3A Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Predicted

Rat, Cow (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

aa89-120

Specificity

This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A.

Applications

IHC
IHC - Paraffin (1:10 - 1:50)
ICC (1:10 - 1:50)
Immunofluorescence (1:100)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAP1LC3A / LC3A

Uniprot: Q9H492 NCBI: NM_032514 NP_115903.1

LSBio Ratings

MAP1LC3A / LC3A Antibody (aa89-120) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C159363 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Autophagy patterns and prognosis in uveal melanomas. Giatromanolaki AN, Charitoudis GS, Bechrakis NE, Kozobolis VP, Koukourakis MI, Foerster MH, Sivridis EL. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 2011 24:1036-45.

PubMed: 21499230

Submit a Publication for LS-C159363