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Catalog Number	Size	Price
LS-C165694-200	200 µl (0.5 mg/ml)	$393

MAP1LC3A / LC3A Antibody - 10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.

MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

MAP1LC3A / LC3A Antibody - Immunofluorescence of U251 cells, using LC3 Antibody (APG8). U251 cells(right) were treated with Chloroquine (50 mu M,16h). The antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

MAP1LC3A / LC3A Antibody - Western blot of anti-LC3 antibody HeLa cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.

$MAP1LC3A / LC3A Antibody - Western blot of anti-LC3 antibody at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).$

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Monoclonal Mouse anti‑Human MAP1LC3A / LC3A Antibody (IHC, IF, WB) LS‑C165694

Name: Anti-MAP1LC3A / LC3A Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C165694-200
Price: 393.0000 USD
Availability: InStock
Rating: 4.1 (1 reviews)

(4.1) Rating

Submit Review (2) Publication(s) LSBio Guarantee

Antibody:

MAP1LC3A / LC3A Mouse anti-Human Monoclonal Antibody

Application:

IHC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$393

LS-C165694-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MAP1LC3A / LC3A Mouse anti-Human Monoclonal Antibody

Application:

IHC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

LC3A antibody LS-C165694 is an unconjugated mouse monoclonal antibody to LC3A (MAP1LC3A) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Cited in 2 publications.

(2) Publication(s)

Target

Human MAP1LC3A / LC3A

Synonyms

See All MAP1LC3A / LC3A Antibodies

Host

Mouse

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Monoclonal

Conjugations

Unconjugated

Purification

Protein G purified

Modifications

Unmodified

Applications

IHC (1:50 - 1:100)
Immunofluorescence (1:200)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAP1LC3A / LC3A

Uniprot: Q9H492 NCBI: NM_032514 NP_115903.1

LSBio Ratings

MAP1LC3A / LC3A Antibody for IHC, IF/Immunofluorescence, WB/Western LS-C165694 has an LSBio Rating of

(4.1) Rating

Publications (4.1)

Learn more about The LSBio Ratings Algorithm

Publications (2)

Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis. Song D, Lee FS. The Journal of biological chemistry. 2011 286:15797-805.

PubMed: 21367862 PMC: PMC3091189

Mitochondria-lysosome membrane contacts are defective in GDAP1-related Charcot-Marie-Tooth disease. Lara Cantarero , Elena Juárez-Escoto , Azahara Civera-Tregón , María Rodríguez-Sanz, Mónica Roldán, Raúl Benítez , Janet Hoenicka , Francesc Palau. The Breast : official journal of the European Society of Mastology. 2021 Jan;29:3589-3605.

Full Text Article PubMed: 33372681 PMC: PMC7823109

Submit a Publication for LS-C165694