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Catalog Number	Size	Price
LS-C165699-400	400 µl	$393

MAP1LC3A / LC3A Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

MAP1LC3A / LC3A Antibody - Fluorescent image of U251 cells stained LC3 (APG8A) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LC3 (APG8A) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

MAP1LC3A / LC3A Antibody - APG8a (MAP1LC3A) Antibody (M1) western blot of mouse brain tissue lysates (35 ug/lane). The APG8a (MAP1LC3A) antibody detected the APG8a (MAP1LC3A) protein (arrow).

$MAP1LC3A / LC3A Antibody - Western blot of anti-LC3 (APG8a) antibody in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).$

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Polyclonal Rabbit anti‑Human MAP1LC3A / LC3A Antibody (aa1‑30, IHC, IF, WB) LS‑C165699

Name: Anti-MAP1LC3A / LC3A Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C165699-400
Price: 393.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

MAP1LC3A / LC3A Rabbit anti-Human Polyclonal (aa1-30) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$393

LS-C165699-400

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(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MAP1LC3A / LC3A Rabbit anti-Human Polyclonal (aa1-30) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

LC3A antibody LS-C165699 is an unconjugated rabbit polyclonal antibody to LC3A (MAP1LC3A) (aa1-30) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human MAP1LC3A / LC3A

Synonyms

See All MAP1LC3A / LC3A Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa1-30

Specificity

This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence (1:200)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAP1LC3A / LC3A

Uniprot: Q9H492 NCBI: NM_032514 NP_115903.1

LSBio Ratings

MAP1LC3A / LC3A Antibody (aa1-30) for IHC, IF/Immunofluorescence, WB/Western LS-C165699 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease. Nascimento-Ferreira I, Santos-Ferreira T, Sousa-Ferreira L, Auregan G, Onofre I, Alves S, Dufour N, Colomer Gould VF, Koeppen A, Dglon N, Pereira de Almeida L. Brain : a journal of neurology. 2011 134:1400-15.

PubMed: 21478185

Submit a Publication for LS-C165699