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Catalog Number Size Price
LS-B15337-50 50 µl $460 
PDI / P4HB Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
PDI / P4HB Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
PDI / P4HB Antibody - Immunofluorescence analysis of HeLa cells using P4HB antibody. Blue: DAPI for nuclear staining.
PDI / P4HB Antibody - Immunofluorescence analysis of HeLa cells.
PDI / P4HB Antibody - Western blot analysis of extracts from normal (control) and P4HB knockout (KO) HeLa cells, using P4HB antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
PDI / P4HB Antibody - Western blot analysis of extracts of various cell lines, using P4HB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PDI / P4HB Antibody - Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody. Western blot was performed from the immunoprecipitate using P4HB antibodyat a dilition of 1:500.
PDI / P4HB Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
PDI / P4HB Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
PDI / P4HB Antibody - Immunofluorescence analysis of HeLa cells using P4HB antibody. Blue: DAPI for nuclear staining.
PDI / P4HB Antibody - Immunofluorescence analysis of HeLa cells.
PDI / P4HB Antibody - Western blot analysis of extracts from normal (control) and P4HB knockout (KO) HeLa cells, using P4HB antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
PDI / P4HB Antibody - Western blot analysis of extracts of various cell lines, using P4HB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PDI / P4HB Antibody - Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody. Western blot was performed from the immunoprecipitate using P4HB antibodyat a dilition of 1:500.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PDI / P4HB Antibody (IHC, IF, WB) LS‑B15337

IHC‑plus™ Polyclonal Rabbit anti‑Human PDI / P4HB Antibody (IHC, IF, WB) LS‑B15337

Note: This antibody replaces LS-C331072
Antibody:
PDI / P4HB Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B15337-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PDI / P4HB Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
P4HB antibody LS-B15337 is an unconjugated rabbit polyclonal antibody to P4HB (PDI) from human. It is reactive with human and mouse. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PDI / P4HB
Synonyms
P4HB | ERBA2L | DSI | GIT | p4HB | p4Hbeta | PDIA1 | PHDB | PO4DB | PROHB | Protocollagen hydroxylase | Protein disulfide-isomerase | PO4HB | PDI
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2). PESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL
Specificity
Human PDI / P4HB
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:20 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 57kDa, while the observed MW by Western blot was 57kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDI / P4HB
P07237 NM_000918 NP_000909.2

Validation

PDI / P4HB Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.

PDI / P4HB Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.

LSBio Ratings

IHC-plus™ PDI / P4HB Antibody for IHC, IF/Immunofluorescence, WB/Western, IP LS-B15337 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024