Human PDI / P4HB

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the sequence around Pro329 of human PDI (KLH).

Recognizes endogenous levels of total human PDI protein. Species cross-reactivity: monkey, mouse and rat.

• IHC
• IHC - Paraffin (1:50)
• ICC (1:50)
• Immunofluorescence (1:50)
• Western blot (1:1000)

Immunohistochemistry: LS-B2372 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2372 was determined to be 1:50. Immunofluorescence (IF-IC): 1:25. Requires methanol permeabilization.

Supplied as a liquid in 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07237 NCBI: NM_000918 NP_000909.2