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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3137-50 | 50 µg (1 mg/ml) | $505 |
1 of 2
2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Rat PDI / P4HB Antibody (aa499‑509, IHC, WB) LS‑B3137
IHC‑plus™ Monoclonal Mouse anti‑Rat PDI / P4HB Antibody (aa499‑509, IHC, WB) LS‑B3137
Note: This antibody replaces LS-C38113
Antibody:
PDI / P4HB Mouse anti-Rat Monoclonal (aa499-509) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PDI / P4HB Mouse anti-Rat Monoclonal (aa499-509) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus
Format:
Unconjugated, Unmodified
Specifications
Description
P4HB antibody LS-B3137 is an unconjugated mouse monoclonal antibody to P4HB (PDI) (aa499-509) from rat. It is reactive with human, mouse, rat and other species. Validated for ICC, IHC and WB. Cited in 1 publication.
Target
Rat PDI / P4HB
Synonyms
P4HB | ERBA2L | DSI | GIT | p4HB | p4Hbeta | PDIA1 | PHDB | PO4DB | PROHB | Protocollagen hydroxylase | Protein disulfide-isomerase | PO4HB | PDI
Host
Mouse
Reactivity
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa499-509 of rat protein disulfide isomerase (KLH coupled).
Epitope
aa499-509
Specificity
Recognizes rat protein disulfide isomerase at ~58kD.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ICC
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3137 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3137 was determined to be 5 ug/ml. Positive control: HeLa Heat Shocked Cell Lysate, MCF-7 cell lysate.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Aliquots are stable for 1 year after receipt.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PDI / P4HB
Validation
Anti-P4HB antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-P4HB antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-P4HB antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ PDI / P4HB Antibody (aa499-509) for IHC, ICC, WB/Western LS-B3137 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Temporal gene expression profiling of the rat knee joint capsule during immobilization-induced joint contractures. Wong K, Sun F, Trudel G, Sebastiani P, Laneuville O. BMC musculoskeletal disorders. 2015 16:125. (ICC; Human, Rat)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus
Applications:
Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
Date Requested: 11/27/2024
