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| Catalog Number | Size | Price |
|---|---|---|
| LS-B109-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human HIF1A / HIF1 Alpha Antibody (clone ESEE122, aa329‑530, IHC, IF, WB) LS‑B109
IHC‑plus™ Monoclonal Mouse anti‑Human HIF1A / HIF1 Alpha Antibody (clone ESEE122, aa329‑530, IHC, IF, WB) LS‑B109
Note: This antibody replaces LS-C2555, LS-C150386
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
HIF1 Alpha antibody LS-B109 is an unconjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) (aa329-530) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
ESEE122
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Human HIF-1 alpha amino acids 329-530.
Epitope
aa329-530
Specificity
HIF-1 alpha.
Applications
- IHC
- IHC - Paraffin (1.25 µg/ml)
- IHC - Frozen (1:100 - 1:5000)
- ICC
- Immunofluorescence (8 - 12 µg/ml)
- Western blot (1:500 - 1:1000)
- Immunoprecipitation (1:10 - 1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B109 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B109 was determined to be 1.25 ug/ml.
Presentation
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIF1A / HIF1 Alpha
Validation
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa329-530, clone ESEE122) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B109 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Species:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Applications:
IHC, IHC - Paraffin
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Dog, Guinea pig, Xenopus, Zebrafish, Primate
Applications:
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Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
