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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2823-0.1 | 0.1 ml (1 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa432‑528, IHC, WB) LS‑B2823
IHC‑plus™ Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa432‑528, IHC, WB) LS‑B2823
Note: This antibody replaces LS-C2559
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
HIF1 Alpha antibody LS-B2823 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa432-528) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Guinea pig, Xenopus, Zebrafish, Primate
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Fusion protein containing amino acids 432-528 of human HIF-1 alpha [UniProt# Q16665]
Epitope
aa432-528
Specificity
HIF-1 alpha.
Applications
- IHC
- IHC - Paraffin (1:100)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2823 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2823 was determined to be 1:100.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIF1A / HIF1 Alpha
Validation
Anti-HIF1A antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa432-528) for IHC, WB/Western LS-B2823 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Hypoxia-independent upregulation of placental hypoxia inducible factor-1a gene expression contributes to the pathogenesis of preeclampsia. Iriyama T, Wang W, Parchim NF, Song A, Blackwell SC, Sibai BM, Kellems RE, Xia Y. Hypertension. 2015 65:1307-15. (IHC, WB; Mouse, Human)
Reciprocal upregulation of hypoxia-inducible factor-1a and persistently enhanced placental adenosine signaling contribute to the pathogenesis of preeclampsia. Takayuki Iriyama, Wei Wang, Nicholas F Parchim, Seisuke Sayama, Keiichi Kumasawa, Takeshi Nagamatsu, Anren Song, Yang Xia, Rodney E Kellems. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2020 March;34:4041-4054.
Customer Reviews (0)
Featured Products
Species:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Applications:
IHC, IHC - Paraffin
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Reactivity:
Rabbit
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
