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| Catalog Number | Size | Price |
|---|---|---|
| LS-B495-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Mouse HIF1A / HIF1 Alpha Antibody (aa530‑825, IHC) LS‑B495
IHC‑plus™ Polyclonal Rabbit anti‑Mouse HIF1A / HIF1 Alpha Antibody (aa530‑825, IHC) LS‑B495
Note: This antibody replaces LS-C2558
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
HIF1 Alpha antibody LS-B495 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa530-825) from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC. Cited in 7 publications.
Target
Mouse HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A fusion protein including aa 530-825 of the mouse HIF-1 alpha protein.
Epitope
aa530-825
Specificity
HIF-1 alpha.
Applications
- IHC
- IHC - Paraffin (5 - 10 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B495 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B495 was determined to be 5-10 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIF1A / HIF1 Alpha
Validation
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa530-825) for IHC LS-B495 has an LSBio Rating of
Publications (4.6)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (7)
Carbon ion radiotherapy of human lung cancer attenuates HIF-1 signaling and acts with considerably enhanced therapeutic efficiency. Subtil FS, Wilhelm J, Bill V, Westholt N, Rudolph S, Fischer J, Scheel S, Seay U, Fournier C, Taucher-Scholz G, Scholz M, Seeger W, Engenhart-Cabillic R, Rose F, Dahm-Daphi J, H[Character e4]nze J. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014 Mar;28:1412-21.
HIF-1 alpha signaling is augmented during intermittent hypoxia by induction of the Nrf2 pathway in NOX1-expressing adenocarcinoma A549 cells. Malec V, Gottschald OR, Li S, Rose F, Seeger W, H[Character e4]nze J. Free radical biology & medicine. 2010 Jun;48:1626-35.
Loss of HIF-1 alpha in the notochord results in cell death and complete disappearance of the nucleus pulposus. Merceron C, Mangiavini L, Robling A, Wilson TL, Giaccia AJ, Shapiro IM, Schipani E, Risbud MV. PloS one. 9:e110768.
ATF4 promotes bone angiogenesis by increasing VEGF expression and release in the bone environment. Zhu K, Jiao H, Li S, Cao H, Galson DL, Zhao Z, Zhao X, Lai Y, Fan J, Im HJ, Chen D, Xiao G. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2013 28:1870-1884. (IHC; Mouse)
Hypoxia-driven Hif2a coordinates mouse liver regeneration by coupling parenchymal growth to vascular expansion. Kron P, Linecker M, Limani P, Schlegel A, Kambakamba P, Lehn JM, Nicolau C, Graf R, Humar B, Clavien PA. Hepatology (Baltimore, Md.). 2016 December;64:2198-2209. (Mouse)
Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway. Komal Qureshi-Baig, Diana Kuhn, Elodie Viry, Vitaly I Pozdeev, Martine Schmitz, Fabien Rodriguez, Pit Ullmann, Eric Koncina, Martin Nurmik, Sonia Frasquilho, Petr V Nazarov, Nikolaus Zuegel, Marc Boulmont, Yervand Karapetyan, Laurent Antunes, Daniel Val, Michel Mittelbronn, Bassam Janji, Serge Haan, Elisabeth Letellier. Autophagy. 2019 Aug;16:1436-1452.
Evolution of ischemia and neovascularization in a murine model of full thickness human wound healing. Aos S Karim, Aiping Liu, Christie Lin, Adam J Uselmann, Kevin W Eliceiri, Matthew E Brown, Angela L F Gibson. Wound repair and regeneration: official publication of the Wound Healing Society [and] the European Tissue Repair Society. 2020 November;28:812-822.
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Featured Products
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Dog, Guinea pig, Xenopus, Zebrafish, Primate
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Reactivity:
Rabbit
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
