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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1503-1 | 1 Each | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa833‑850, IHC, WB) LS‑B1503
IHC‑plus™ Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa833‑850, IHC, WB) LS‑B1503
Note: This antibody replaces LS-C11695
Antibody:
HIF1A / HIF1 Alpha Rabbit anti-Human Polyclonal (aa833-850) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
HIF1A / HIF1 Alpha Rabbit anti-Human Polyclonal (aa833-850) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Format:
Unconjugated, Unmodified
Specifications
Description
HIF1 Alpha antibody LS-B1503 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa833-850) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
(tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
HIF-1 amino acids 809-826. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%); Turkey, Chicken, Platypus (94%); Opossum, Lizard, Xenopus (89%); Pike, Zebrafish (83%).
Epitope
aa833-850
Specificity
HIF-1 alpha amino acids (SRNLLQGEELLRALDQVN)
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1503 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1503 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.2, 0.02% Sodium Azide, 50% Glycerol
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIF1A / HIF1 Alpha
Validation
Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa833-850) for IHC, WB/Western LS-B1503 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Loss of PINK1 attenuates HIF-1 alpha induction by preventing 4E-BP1-dependent switch in protein translation under hypoxia. Lin W, Wadlington NL, Chen L, Zhuang X, Brorson JR, Kang UJ. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2014 Feb;34:3079-89.
Biopolymer-delivered vascular endothelial growth factor improves renal outcomes following revascularization. Erika Guise, Jason E Engel, Maxx L Williams, Fakhri Mahdi, Gene L Bidwell rd, Alejandro R Chade. American journal of physiology. Renal physiology. 2019 May;316:F1016-F1025.
Customer Reviews (0)
Featured Products
Species:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat, Dog, Guinea pig, Xenopus, Zebrafish, Primate
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Reactivity:
Rabbit
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
