Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Location
Corporate Headquarters
Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States
Telephone Numbers
Customer Service: (800) 227-6666 / (650) 697-3600
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-C7464-100 | 100 µl | $688 |
Monoclonal Mouse anti‑Human HIF1A / HIF1 Alpha Antibody (aa432‑528, IHC, WB) LS‑C7464
Monoclonal Mouse anti‑Human HIF1A / HIF1 Alpha Antibody (aa432‑528, IHC, WB) LS‑C7464
Antibody:
HIF1A / HIF1 Alpha Mouse anti-Human Monoclonal (aa432-528) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Sheep
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
HIF1A / HIF1 Alpha Mouse anti-Human Monoclonal (aa432-528) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Sheep
Format:
Unconjugated, Unmodified
Specifications
Description
HIF1 Alpha antibody LS-C7464 is an unconjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) (aa432-528) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Pig, Sheep
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Fusion protein of human HIF-1 alpha, aa432-528. Cellular Localization: Nuclear.
Epitope
aa432-528
Specificity
Recognizes human HIF-1 alpha at 120kD. Species cross-reactivity: monkey, sheep, mouse, rat, porcine, bovine and ferret.
Applications
- IHC
- IHC - Paraffin
- Western blot (1:250)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Western Blot: 1:250 (ECL, nuclear extracts). Recognizes a band at 120kD representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen representing post-translational modification. The mobility of HIF-1 alpha induced by desferrioxamine or cobalt chloride treatment differs from the mobility of the hypoxia-induced protein. The reason is not known. Immunohistochemistry (paraffin tissues): 1:250-1:500. Positive control: Western Blot: COS-7 treated and untreated nuclear extracts can be used for positive and negative controls for hypoxic upregulation.
Presentation
PBS, pH 7.2, 0.05% Sodium Azide, 40% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIF1A / HIF1 Alpha
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Applications:
IHC, IHC - Paraffin
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Dog, Guinea pig, Xenopus, Zebrafish, Primate
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
