Human FN1 / Fibronectin

Rabbit

Human, Monkey, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin

Affinity chromatography

Unmodified

Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.

Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

• IHC (1:50 - 1:200)
• Western blot (1:500 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:20000)

Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 ug of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02751 NCBI: NM_002026 NP_002017.1