Human FN1 / Fibronectin

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity chromatography

Unmodified

Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.

Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

• IHC
• IHC - Paraffin (1.25 µg/ml)
• Western blot (1:5000 - 1:10000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:10000)

Immunohistochemistry: LS-B382 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B382 was determined to be 1.25 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02751 NCBI: NM_002026 NP_002017.1