Human FN1 / Fibronectin

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin. Also available conjugated with Biotin, APC, CF.

Immunoaffinity purified

Unmodified

Fibronectin purified from human plasma.

Recognizes human Fibronectin. Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reactivity with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

• IHC
• IHC - Paraffin
• Western blot (1:5000 - 1:10000)
• Immunoprecipitation
• ELISA (1:4000 - 1:8000)
• Applications tested for the base form of this product only

Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. ELISA: 1:4000-1:8000. Assayed against 1 ug of Fibronectin in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT. Immunohistochemistry (Paraffin embedded tissue): 1:50-1:200. Western Blot: 1:5000-1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/ml BSA (IgG, Protease free), PBS, pH 7.2, 0.01% Sodium Azide

100 ul sterile ddH20

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02751 NCBI: NM_002026 NP_002017.1