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Catalog Number Size Price
LS-C166866-400 400 µl $393 
HIF1A / HIF1 Alpha Antibody - HIF1A Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
HIF1A / HIF1 Alpha Antibody - Fluorescent confocal image of HeLa cell stained with HIF1A Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIF1A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HIF1A immunoreactivity is localized to cytoplasm and nucleus significantly.
HIF1A / HIF1 Alpha Antibody - Western blot of HIF1A Antibody in CHO cell line lysates (35 ug/lane). HIF1A (arrow) was detected using the purified antibody.
HIF1A / HIF1 Alpha Antibody - HIF1A Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
HIF1A / HIF1 Alpha Antibody - Fluorescent confocal image of HeLa cell stained with HIF1A Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIF1A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HIF1A immunoreactivity is localized to cytoplasm and nucleus significantly.
HIF1A / HIF1 Alpha Antibody - Western blot of HIF1A Antibody in CHO cell line lysates (35 ug/lane). HIF1A (arrow) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa1‑30, IHC, IF, WB) LS‑C166866

Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa1‑30, IHC, IF, WB) LS‑C166866

Antibody:
HIF1A / HIF1 Alpha Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C166866-400
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HIF1A / HIF1 Alpha Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
HIF1 Alpha antibody LS-C166866 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa1-30) from human. It is reactive with human and hamster. Validated for IF, IHC and WB.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Human, Hamster (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa1-30
Specificity
This HIF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIF1A.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

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Requested From: United States
Date Requested: 12/1/2024