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Antibodies > ALOX5 / 5-LOX > LS-C806387
 
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Catalog Number Size Price
LS-C806387-100 100 µl (1 mg/ml) $379 
LS-C806387-200 200 µl (1 mg/ml) $421 
ALOX5 / 5-LOX Antibody - 1:100 staining human brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ALOX5 / 5-LOX Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ALOX5 / 5-LOX Antibody - Western blot analysis of extracts of HepG2 cells using ALOX5 antibody. The lane on the left is treated with the antigen-specific peptide.
ALOX5 / 5-LOX Antibody - 1:100 staining human brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ALOX5 / 5-LOX Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ALOX5 / 5-LOX Antibody - Western blot analysis of extracts of HepG2 cells using ALOX5 antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human ALOX5 / 5‑LOX Antibody (IHC, IF, WB) LS‑C806387

Polyclonal Rabbit anti‑Human ALOX5 / 5‑LOX Antibody (IHC, IF, WB) LS‑C806387

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Antibody:
ALOX5 / 5-LOX Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C806387-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ALOX5 / 5-LOX Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
5-LOX antibody LS-C806387 is an unconjugated rabbit polyclonal antibody to 5-LOX (ALOX5) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human ALOX5 / 5-LOX
Synonyms
ALOX5 | 5-lipoxygenase | 5LPG | 5-LO | 5-LOX | Arachidonate 5-lipoxygenase | LOG5 | LOX-5 | Leukotriene A4 synthase
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human ALOX5.
Specificity
ALOX5 Antibody detects endogenous levels of total ALOX5.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALOX5 / 5-LOX
P09917 NM_000698 NP_000689.1

Publications (0)

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Featured Products

ALOX5 / 5-LOX Antibody - Anti-5 Lipoxygenase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ ALOX5 / 5‑LOX Antibody (aa130‑149) LS‑B1499
Species: Human, Rat, Bovine
Applications: IHC, IHC - Paraffin, ICC, Western blot
ALOX5 / 5-LOX Antibody - Anti-5 Lipoxygenase antibody IHC of human spleen, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC‑plus™ ALOX5 / 5‑LOX Antibody (aa600‑674) LS‑B2164
Species: Human, Mouse, Rat, Bovine, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
ALOX5 / 5-LOX Antibody - Anti-5 Lipoxygenase antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC‑plus™ ALOX5 / 5‑LOX Antibody (aa100‑200) LS‑B2144
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
ALOX5 / 5-LOX Antibody - Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Arachidonate 5 Lipoxygenase (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide.
ALOX5 / 5‑LOX Antibody (phospho‑Ser271) LS‑C198639
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
ALOX5 / 5-LOX Antibody - Western blot of Human HepG2. ALOX5 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ALOX5 / 5‑LOX Antibody (C‑Terminus) LS‑C201775
Species: Human
Applications: Western blot
ALOX5 / 5-LOX ELISA Kit
Mouse ALOX5 / 5-LOX (Sandwich ELISA) ELISA Kit - LS-F11968
Reactivity: Mouse
Range: 0.156-10 ng/ml
See all ALOX5 / 5-LOX Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
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