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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2144-0.1 | 0.1 ml (1 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human ALOX5 / 5‑LOX Antibody (aa100‑200, IHC, WB) LS‑B2144
IHC‑plus™ Polyclonal Rabbit anti‑Human ALOX5 / 5‑LOX Antibody (aa100‑200, IHC, WB) LS‑B2144
Note: This antibody replaces LS-C32005
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
5-LOX antibody LS-B2144 is an unconjugated rabbit polyclonal antibody to 5-LOX (ALOX5) (aa100-200) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ALOX5 / 5-LOX
Synonyms
ALOX5 | 5-lipoxygenase | 5LPG | 5-LO | 5-LOX | Arachidonate 5-lipoxygenase | LOG5 | LOX-5 | Leukotriene A4 synthase
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide made to a region within residues 100-200 of the human protein. [Swiss-Prot# P09917]
Epitope
aa100-200
Specificity
LOX5.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2144 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2144 was determined to be 5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ALOX5 / 5-LOX
Validation
Anti-5 Lipoxygenase antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-5 Lipoxygenase antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ ALOX5 / 5-LOX Antibody (aa100-200) for IHC, WB/Western LS-B2144 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Rat, Bovine
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Human, Mouse, Rat, Bovine, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
