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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1499-1 | 1 Each | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human ALOX5 / 5‑LOX Antibody (aa130‑149, IHC, WB) LS‑B1499
IHC‑plus™ Polyclonal Rabbit anti‑Human ALOX5 / 5‑LOX Antibody (aa130‑149, IHC, WB) LS‑B1499
Note: This antibody replaces LS-C11173
Antibody:
ALOX5 / 5-LOX Rabbit anti-Human Polyclonal (aa130-149) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Rat, Bovine
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
ALOX5 / 5-LOX Rabbit anti-Human Polyclonal (aa130-149) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Rat, Bovine
Format:
Unconjugated, Unmodified
Specifications
Description
5-LOX antibody LS-B1499 is an unconjugated rabbit polyclonal antibody to 5-LOX (ALOX5) (aa130-149) from human. It is reactive with human, rat and bovine. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ALOX5 / 5-LOX
Synonyms
ALOX5 | 5-lipoxygenase | 5LPG | 5-LO | 5-LOX | Arachidonate 5-lipoxygenase | LOG5 | LOX-5 | Leukotriene A4 synthase
Host
Rabbit
Reactivity
Human, Rat, Bovine
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Bat, Hamster, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Human and rat amino acids 130-149 (QHRRKELETRQKQYRWMEWN). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Rat, Bovine, Elephant, Panda (100%); Monkey, Mouse, Hamster, Rabbit, Opossum (95%); Bat (90%); Turkey, Chicken, Salmon, Zebrafish (80%).
Epitope
aa130-149
Specificity
Human and rat amino acids 130-149 (QHRRKELETRQKQYRWMEWN); The sequence is 95% homologous to 5-LO from mouse and hamster
Applications
- IHC
- IHC - Paraffin (1:50 - 1:100)
- ICC
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1499 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1499 was determined to be 10 ug/ml.
Presentation
TBS, pH 7.4
Reconstitution
500 ul sterile ddH20
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ALOX5 / 5-LOX
Validation
Anti-5 Lipoxygenase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-5 Lipoxygenase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ALOX5 / 5-LOX Antibody (aa130-149) for IHC, ICC, WB/Western LS-B1499 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse, Rat, Bovine, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
