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Catalog Number Size Price
LS-C806737-100 100 µl (1 mg/ml) $379 
LS-C806737-200 200 µl (1 mg/ml) $421 
ACAN / Aggrecan Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ACAN / Aggrecan Antibody - Western blot analysis of Aggrecan using 293 whole cells lysates
ACAN / Aggrecan Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ACAN / Aggrecan Antibody - Western blot analysis of Aggrecan using 293 whole cells lysates
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Polyclonal Rabbit anti‑Human ACAN / Aggrecan Antibody (IF, WB) LS‑C806737

Polyclonal Rabbit anti‑Human ACAN / Aggrecan Antibody (IF, WB) LS‑C806737

Antibody:
ACAN / Aggrecan Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C806737-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ACAN / Aggrecan Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Aggrecan antibody LS-C806737 is an unconjugated rabbit polyclonal antibody to Aggrecan (ACAN) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human ACAN / Aggrecan
Synonyms
ACAN | AGCAN | Aggrecan core protein | Acg1 | Aggrecan | CSPG1 | CSPGCP | CSPCP | AGC1 | Aggrecan 1 | Large aggregating proteoglycan | MSK16 | SEDK | MCSPG
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human Aggrecan
Specificity
Aggrecan Antibody detects endogenous levels of total Aggrecan.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACAN / Aggrecan
P16112 NM_013227 NP_037359.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024