Human ACAN / Aggrecan

Rabbit

Human, Monkey, Hamster, Pig (tested or 100% immunogen sequence identity)

Mouse, Rat, Bovine, Horse (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic 18 amino acid peptide from N-Terminus of human ACAN / Aggrecan. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Hamster, Pig (100%); Mouse, Rat, Bovine, Panda, Horse (94%); Dog, Elephant (89%); Bat (83%).

N-Terminus

Human ACAN / Aggrecan. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NCAN (56%).

• IHC
• IHC - Paraffin (7 µg/ml)

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml.

PBS, 0.1% Sodium Azide

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16112 NCBI: NM_013227 NP_037359.3