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| Catalog Number | Size | Price |
|---|---|---|
| LS-A1556-50 | 50 µg (1 mg/ml) | $440 |
IHC‑plus™ Polyclonal Rabbit anti‑Human ACAN / Aggrecan Antibody (N‑Terminus, IHC) LS‑A1556
IHC‑plus™ Polyclonal Rabbit anti‑Human ACAN / Aggrecan Antibody (N‑Terminus, IHC) LS‑A1556
Antibody:
ACAN / Aggrecan Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Hamster, Pig
Format:
Unconjugated, Unmodified
Overview
Antibody:
ACAN / Aggrecan Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Hamster, Pig
Format:
Unconjugated, Unmodified
Specifications
Description
Aggrecan antibody LS-A1556 is an unconjugated rabbit polyclonal antibody to Aggrecan (ACAN) (N-Terminus) from human. It is reactive with human, hamster, monkey and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human ACAN / Aggrecan
Synonyms
ACAN | AGCAN | Aggrecan core protein | Acg1 | Aggrecan | CSPG1 | CSPGCP | CSPCP | AGC1 | Aggrecan 1 | Large aggregating proteoglycan | MSK16 | SEDK | MCSPG
Host
Rabbit
Reactivity
Human, Monkey, Hamster, Pig
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 18 amino acid peptide from N-Terminus of human ACAN / Aggrecan. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Hamster, Pig (100%); Mouse, Rat, Bovine, Panda, Horse (94%); Dog, Elephant (89%); Bat (83%).
Epitope
N-Terminus
Specificity
Human ACAN / Aggrecan. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NCAN (56%).
Applications
- IHC
- IHC - Paraffin (7 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ACAN / Aggrecan
Validation
Anti-ACAN / Aggrecan antibody IHC of human bone, cartilage. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-ACAN / Aggrecan antibody IHC of human bone, cartilage. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
IHC-plus™ ACAN / Aggrecan Antibody (N-Terminus) for IHC LS-A1556 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin
Species:
Human, Mouse, Rat, Bovine, Dog, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Sheep
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Rat, Hamster
Applications:
IHC, IHC - Paraffin
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
Date Requested: 12/25/2024
