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fliC / Flagellin

Flagellin (E. coli)

Gene Name: Flagellin (E. coli)
Synonyms: fliC
Target Sequences: NP_416433.1

Publications (7)

1
Salmonella virulence factor SpiC is involved in expression of flagellin protein and mediates activation of the signal transduction pathways in macrophages. Uchiya K, Nikai T. Microbiology (Reading, England). 2008 154:3491-502. [PubMed:18957602]
2
Use of toll-like receptor assays to detect and identify microbial contaminants in biological products. Huang LY, Dumontelle JL, Zolodz M, Deora A, Mozier NM, Golding B. Journal of clinical microbiology. 2009 47:3427-34. [PubMed:19726599] [PMC:PMC2772586]
3
Induction of Salmonella pathogenicity island 1 under different growth conditions can affect Salmonella-host cell interactions in vitro. Ibarra JA, Knodler LA, Sturdevant DE, Virtaneva K, Carmody AB, Fischer ER, Porcella SF, Steele-Mortimer O. Microbiology (Reading, England). 2010 156:1120-33. [PubMed:20035008] [PMC:PMC2848694]
4
Flagellated but not hyperfimbriated Salmonella enterica serovar Typhimurium attaches to and forms biofilms on cholesterol-coated surfaces. Crawford RW, Reeve KE, Gunn JS. Journal of bacteriology. 2010 192:2981-90. [PubMed:20118264] [PMC:PMC2901699]
5
Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia. Knodler LA, Vallance BA, Celli J, Winfree S, Hansen B, Montero M, Steele-Mortimer O. Proceedings of the National Academy of Sciences of the United States of America. 2010 107:17733-8. [PubMed:20876119] [PMC:PMC2955089]
6
Dissimilar properties of two recombinant Lactobacillus acidophilus strains displaying Salmonella FliC with different anchoring motifs. Kajikawa A, Nordone SK, Zhang L, Stoeker LL, LaVoy AS, Klaenhammer TR, Dean GA. Applied and environmental microbiology. 2011 77:6587-96. [PubMed:21784918] [PMC:PMC3187123]
7
Effect of iacP mutation on flagellar phase variation in Salmonella enterica serovar typhimurium strain UK-1. Eom JS, Kim JS, Jang JI, Kim HG, Bang IS, Park YK. Journal of bacteriology. 2012 194:4332-41. [PubMed:22685287] [PMC:PMC3416257]
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☰ Filters
Antibodies (15)
Proteins (10)
Native (2)
Primary (15)
Recombinant (8)
fliC / Flagellin (25)
Bacteria (1)
Borrelia burgdorferi (10)
E. coli (3)
Escherichia coli (1)
WB (4)
ELISA (15)
IF (5)
rabbit (10)
mouse (5)
IgG (10)
IgG2a (5)
monoclonal mc (5)
polyclonal pc (10)
6802 (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
Liquid (1)
Lyophilized (1)
PE Conjugated (1)
Unconjugated (5)
aa1-336 (4)
No (25)
6His, N-terminus (1)
6His-SUMO, N-terminus (2)
His (2)
His, N-terminus (1)
Clostridium botulinum (1)
E. coli (1)
Escherichia coli (1)
Salmonella enterica (3)
Salmonella typhimurium (1)
E. coli (8)
Salmonella typhimurium (2)
Purified (6)
Low endotoxin level (5)
Select
fliC / Flagellin Rabbit anti-E. coli Polyclonal Antibody
Escherichia coli
ELISA
Unconjugated
50 µg/$294; 100 µg/$360
Select
fliC / Flagellin Rabbit anti-E. coli Polyclonal (HRP) Antibody
E. coli
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
fliC / Flagellin Rabbit anti-E. coli Polyclonal (Biotin) Antibody
E. coli
ELISA, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
fliC / Flagellin Rabbit anti-E. coli Polyclonal (FITC) Antibody
E. coli
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
fliC / Flagellin Protein - Flagellin (AG-40B-0095) activates TLR5. Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
Select
Salmonella typhimurium
None
Less than 0.1 EU/µg protein (determined by LAL method).
100 µg/$379
fliC / Flagellin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
None
51.2 kDa
1 mg/$1,756; 100 µg/$734; 20 µg/$488
fliC / Flagellin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
Select
fliC / Flagellin Mouse anti-Bacteria Monoclonal (6802) Antibody
Bacteria
ELISA, IF
Unconjugated
0.2 mg/$486
fliC / Flagellin Antibody - Anti-Flagellin Antibody - Western Blot. Western blot showing detection of 0.1 ug of recombinant Flagellin protein. Lane 1: Molecular weight markers. Lane 2: MBP-Flagellin fusion protein (arrowhead at expected MW: 76.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n LS-C60865) secondary antibody was used at 1:40000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Select
fliC / Flagellin Rabbit anti-Borrelia burgdorferi Polyclonal (Lyophilized) Antibody
Borrelia burgdorferi
ELISA, WB
Unconjugated, Lyophilized
100 µg/$567
Select
E. coli
His, N-terminus
Less than 0.01 EU/µg protein (determined by LAL method).
10 µg/$337
fliC / Flagellin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
None
52.3 kDa
1 mg/$1,756; 100 µg/$611; 20 µg/$447
fliC / Flagellin Protein - Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).
Select
Salmonella typhimurium
None
Less than 0.01 EU/µg protein (determined by LAL method).
10 µg/$337
Select
fliC / Flagellin Mouse anti-Borrelia burgdorferi Monoclonal (HRP) Antibody
Borrelia burgdorferi
ELISA, IF
HRP Conjugated
100 µl/$951
Select
fliC / Flagellin Mouse anti-Borrelia burgdorferi Monoclonal (Biotin) Antibody
Borrelia burgdorferi
ELISA, IF
Biotin Conjugated
100 µl/$951
Select
fliC / Flagellin Mouse anti-Borrelia burgdorferi Monoclonal (FITC) Antibody
Borrelia burgdorferi
ELISA, IF
FITC Conjugated
100 µl/$951
Select
fliC / Flagellin Mouse anti-Borrelia burgdorferi Monoclonal (PE) Antibody
Borrelia burgdorferi
ELISA, IF
PE Conjugated
100 µl/$951
fliC / Flagellin Antibody - Western blot showing detection of 0.1 µg of recombinant Flagellin protein. Lane 1: Molecular weight markers. Lane 2: MBP-Flagellin fusion protein (arrowhead at expected MW: 76.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Select
fliC / Flagellin Rabbit anti-Borrelia burgdorferi Polyclonal (Liquid) Antibody
Borrelia burgdorferi
ELISA, WB
Unconjugated, Liquid
25 µl/$304
Select
fliC / Flagellin Rabbit anti-Borrelia burgdorferi Polyclonal (aa1-336) Antibody
Borrelia burgdorferi
ELISA
Unconjugated
50 µg/$294; 100 µg/$360
Select
fliC / Flagellin Rabbit anti-Borrelia burgdorferi Polyclonal (aa1-336) (HRP) Antibody
Borrelia burgdorferi
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
fliC / Flagellin Rabbit anti-Borrelia burgdorferi Polyclonal (aa1-336) (Biotin) Antibody
Borrelia burgdorferi
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
fliC / Flagellin Rabbit anti-Borrelia burgdorferi Polyclonal (aa1-336) (FITC) Antibody
Borrelia burgdorferi
ELISA
FITC Conjugated
50 µg/$294; 100 µg/$360
fliC / Flagellin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His-SUMO, N-terminus
20 µg/$405; 100 µg/$598; 1 mg/$1,679
fliC / Flagellin Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His, N-terminus
56.3 kDa
1 mg/$1,679; 100 µg/$500; 20 µg/$364
Select
E. coli
His
Less than 0.01 EU/µg protein (determined by LAL method).
10 µg/$337
Select
E. coli
His
Less than 0.01 EU/µg protein (determined by LAL method).
10 µg/$337
Viewing 1-25 of 25 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).