Conjugations
Unconjugated
Expression System
E. coli
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at a concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1µg/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages. It can be used as negative control for inflammasome signaling.
Endotoxin
Less than 0.01 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with 100 µl sterile water.
Storage
Store lyophilized at -20°C for up to 6 months. Once reconstituted store at 4°C for immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee