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| Catalog Number | Size | Price |
|---|---|---|
| LS-G3927-100 | 100 µg | $379 |
Salmonella typhimurium fliC / Flagellin Protein (Native) - LS-G3927
Salmonella typhimurium fliC / Flagellin Protein (Native) - LS-G3927
Description:
fliC / Flagellin Protein LS-G3927 is a Native Salmonella typhimurium fliC / Flagellin P06179. It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
fliC / Flagellin Protein LS-G3927 is a Native Salmonella typhimurium fliC / Flagellin P06179. It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Native Protein
Target
fliC / Flagellin
Synonyms
fliC
Species
Salmonella typhimurium
Modifications
Unmodified
Conjugations
Unconjugated
Region
P06179
Source Species
Salmonella typhimurium
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with 100 µl sterile water.
Storage
Store lyophilized at -20°C for up to 6 months. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About fliC / Flagellin
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Functional Assay
Flagellin (AG-40B-0095) activates TLR5. Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
Functional Assay
Flagellin (AG-40B-0095) activates TLR5. Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
Functional Assay
Flagellin (AG-40B-0095) activates TLR5. Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
Functional Assay
Flagellin (AG-40B-0095) activates TLR5. Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
Popular fliC / Flagellin Proteins
Source:
E. coli
Tag:
6His-SUMO, N-terminus
Source:
E. coli
Tag:
None
Source:
E. coli
Tag:
His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/17/2024
Date Requested: 11/17/2024
