Target
Borrelia burgdorferi fliC / Flagellin
Reactivity
Borrelia burgdorferi
(tested or 100% immunogen sequence identity)
Predicted
Borrelia garinii (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Lyophilized
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi Flagellin protein.
Specificity
This antibody is specific for Lyme Borrelia spp. Flagellin protein. A BLAST analysis was used to suggest cross-reactivity with Flagellin from B. burgdorferi, garinii, and valaisiana sources based on 100% homology with the immunizing sequence.. Cross-reactivity with Flagellin from other sources has not been determined.
Applications
- Western blot (1:1000)
- ELISA (1:6000)
Usage
This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 33.9 kD in size corresponding to Borrelia burgdorferi Flagellin protein by Western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee