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DNASE1 / DNase I

deoxyribonuclease I

DNASE1 / DNase I is a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Gene Name: deoxyribonuclease I
Synonyms: DNASE1, Deoxyribonuclease I, Deoxyribonuclease i precursor, DNase I, DNL1, DRNI, Deoxyribonuclease-1, DNase I, lysosomal
Target Sequences: NM_005223 NP_005214.2 P24855

Publications (5)

1
TNFα Amplifies DNaseI Expression in Renal Tubular Cells while IL-1β Promotes Nuclear DNaseI Translocation in an Endonuclease-Inactive Form. Thiyagarajan D, Rekvig OP, Seredkina N. PloS one. 2015 10:e0129485. (IHC, WB; Mouse, Human) [Full Text Article] [PubMed:26065428] [PMC:PMC4465975] Related Antibodies: LS-B4846.
2
IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Thiyagarajan D, Pedersen HL, Seredkina N, Horvei KD, Arranz L, Sonneveld R, Nijenhuis T, van der Vlag J, Rekvig OP. Frontiers in cell and developmental biology. 2018 February;6:7. (Human) [Full Text Article] [PubMed:29468159] [PMC:PMC5807897] Related Antibodies: LS-B4846.
3
Lupus nephritis: low urinary DNase I levels reflect loss of renal DNase I and may be utilized as a biomarker of disease progression. Pedersen HL, Horvei KD, Thiyagarajan D, Norby GE, Seredkina N, Moroni G, Eilertsen GØ, Holdaas H, Strøm EH, Bakland G, Meroni PL, Rekvig OP. The journal of pathology Clinical research. 2018 July;4:193-203. [Full Text Article] [PubMed:29624903] [PMC:PMC6065113] Related Antibodies: LS-B4846.
4
Neutrophil extracellular trap formation in the Streptococcus suis-infected cerebrospinal fluid compartment. Nicole de Buhr, Friederike Reuner, Ariane Neumann, Carolin Stump-Guthier, Tobias Tenenbaum, Horst Schroten, Hiroshi Ishikawa, Kristin Müller, Andreas Beineke, Isabel Hennig-Pauka, Thomas Gutsmann, Peter Valentin-Weigand, Christoph G Baums, Maren von Köckritz-Blickwede. Cellular microbiology. 2017 February;19: [Full Text Article] [PubMed:27450700]
5
Neutrophil Extracellular Traps Profiles in Patients with Incident Systemic Lupus Erythematosus and Lupus Nephritis. Maurizio Bruschi, Alice Bonanni, Andrea Petretto, Augusto Vaglio, Federico Pratesi, Laura Santucci, Paola Migliorini, Roberta Bertelli, Maricla Galetti, Silvana Belletti, Lorenzo Cavagna, Gabriella Moroni, Franco Franceschini, Micaela Fredi, Giulia Pazzola, Landino Allegri, Renato Alberto Sinico, Giampaola Pesce, Marcello Bagnasco, Angelo Manfredi, Giuseppe A Ramirez, Paola Ramoino, Paolo Bianchini, Francesco Puppo, Francesca Pupo, Simone Negrini, Federico Mattana, Giacomo Emmi, Giacomo Garibott. The Journal of rheumatology. 2020 Mar;47:377-386. [Full Text Article] [PubMed:31092713] [PMC:PMC6917988]
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Antibodies (52)
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DNASE1 / DNase I Antibody - Anti-DNase I antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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DNASE1 / DNase I Rabbit anti-Human Polyclonal Antibody
Human
ICC, IHC, IHC-P, WB
Unconjugated
50 µl/$460
DNASE1 / DNase I Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
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DNASE1 / DNase I Mouse anti-Human Monoclonal (aa19-259) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$460
DNASE1 / DNase I Antibody - Immunofluorescence staining of DNASE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human DNASE1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
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DNASE1 / DNase I Rabbit anti-Human Monoclonal (Preservative Free) (006) Antibody
Human
ELISA, ICC, IF
Unconjugated, Preservative Free
20 µl/$271; 100 µl/$412
DNASE1 / DNase I Antibody - Western Blot; Sample: Recombinant protein.
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DNASE1 / DNase I Rabbit anti-Human Polyclonal (aa19-259) Antibody
Mouse, Human
ICC, IP, WB
Unconjugated
100 µl/$303; 200 µl/$343; 500 µl/$476; 1 ml/$543; 2 ml/$715; 5 ml/$1,182
DNASE1 / DNase I Antibody - Western Blot; Sample: Recombinant protein.
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DNASE1 / DNase I Rabbit anti-Human Polyclonal (aa19-259) (Biotin) Antibody
Mouse, Human
WB
Biotin Conjugated
100 µl/$313; 200 µl/$357; 500 µl/$503; 1 ml/$576; 2 ml/$788; 5 ml/$1,279
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DNASE1 / DNase I Goat anti-Bovine Polyclonal Antibody
Bovine
ELISA, WB
Unconjugated
100 µl/$832
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Strip Plate
Human
78 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Saliva, Seminal Plasma, Serum, Urine
1 Plate/$798
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Strip Plate
Bovine
15.6 - 1000 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$816
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$669
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
78 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$669
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Microplate
Human
0.16 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$651
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Microplate
Mouse
1.25 - 80 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$651
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$587
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Strip Plate
Bovine
12.5 - 800 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$1,041
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DNASE1 / DNase I Rabbit anti-Bovine Polyclonal (Liquid) (Biotin) Antibody
Bovine
ELISA, IP, WB
Biotin Conjugated, Liquid
25 µl/$304
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DNASE1 / DNase I Rabbit anti-Bovine Polyclonal (Lyophilized) (Biotin) Antibody
Bovine
ELISA, IP, WB
Biotin Conjugated, Lyophilized
100 µg/$552
DNASE1 / DNase I Antibody - Western Blot;Sample: Recombinant DNASE1, Human.
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DNASE1 / DNase I Mouse anti-Human Monoclonal Antibody
Human
WB
Unconjugated
100 µl/$305; 200 µl/$346; 500 µl/$482; 1 ml/$550; 2 ml/$731; 5 ml/$1,200
DNASE1 / DNase I Antibody - Western blot of recombinant DNASE1 / DNase I.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DNASE1 / DNase I Mouse anti-Human Monoclonal (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$315; 200 µl/$360; 500 µl/$509; 1 ml/$584; 2 ml/$804; 5 ml/$1,305
DNASE1 / DNase I Antibody - DNASE1 / DNase I antibody Western blot of PANC1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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DNASE1 / DNase I Rabbit anti-Human Polyclonal (aa71-120) Antibody
Human
WB
Unconjugated
100 µl/$424
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
1.25 - 80 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$579
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DNASE1 / DNase I Rabbit anti-Bovine Polyclonal Antibody
Bovine
ELISA, IHC, IP, WB
Unconjugated
2 ml/$1,225
DNASE1 / DNase I Antibody - Immunofluorescence analysis of HeLa cell using DNASE1 antibody.
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DNASE1 / DNase I Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, WB
Unconjugated
20 µl/$275; 100 µl/$389
DNASE1 / DNase I Antibody
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DNASE1 / DNase I Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$286; 100 µl/$321
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DNASE1 / DNase I Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
DNASE1 / DNase I ELISA Kit
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Sandwich
96-Well Strip Plate
Pig
31.2 - 2000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$669
Viewing 1-25 of 83 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).