Bovine DNASE1 / DNase I

Rabbit

Bovine (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Lyophilized

Deoxyribonuclease I [Bovine Pancreas].

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas]. Cross reactivities against Deoxyribonuclease I from other sources may occur but have not been specifically determined.

• Western blot (1:500 - 1:5000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:20000)

Anti-Deoxyribonuclease I has been assayed against 1.0 ug of Deoxyribonuclease I in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2000 to 1:8000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P24855 NCBI: NM_005223 NP_005214.2