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CD247 / CD3 Zeta

CD247 molecule

CD247 / CD3 Zeta is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: CD247 molecule
Synonyms: CD247, CD3 Zeta, CD3H, CD3Z, CD247 antigen, zeta subunit, CD247 molecule, CD3zeta chain, T3Z, TCR zeta chain, TCRZ, T-cell receptor T3 zeta chain, CD247 antigen, CD3-ZETA, CD3Q
Target Sequences: NM_000734 NP_000725.1 P20963

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Products
Antibodies (67)
Primary (67)
CD247 / CD3 Zeta (67)
Human (60)
Mouse (30)
Rat (18)
Monkey (3)
Pig (1)
Rabbit (1)
IHC (12)
IHC-P (2)
WB (58)
Flo (18)
ELISA (31)
ICC (2)
IF (10)
IHC-IF (1)
IP (7)
Peptide-ELISA (7)
rabbit (51)
mouse (10)
goat (1)
hamster (5)
Product Group
IHCPlus (2)
IgG (45)
IgG1 (6)
IgG1,k (1)
IgG2a (2)
IgG2b (1)
monoclonal mc (15)
polyclonal pc (51)
recombinant monoclonal rmc (1)
145-2C11 (2)
4A12-F6 (1)
4B10 (2)
EM-17 (1)
EM-26 (1)
EM-54 (1)
EM-55 (1)
H146-968 (3)
APC Conjugated (3)
Azide-free (2)
Biotin Conjugated (6)
FITC Conjugated (8)
HRP Conjugated (5)
PE Conjugated (3)
Preservative Free (1)
Unconjugated (42)
aa113-141 (7)
aa2-31 (7)
pTyr142 (5)
C-Terminus (4)
80-160 aa (2)
aa114-164 (2)
Internal (1)
Internal Region (1)
aa111-160 (1)
aa52-164 (1)
aa75-125 (1)
p42 (1)
pTyr111 (1)
pTyr153 (1)
pTyr72 (1)
No (67)
CD247 / CD3 Zeta Antibody - Anti-CD247 / CD3-Zeta antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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CD247 / CD3 Zeta Mouse anti-Human Monoclonal (aa52-164) (4B10) Antibody
Human
ELISA, Flo, IF, IHC, IHC-P, WB
Unconjugated
50 µl/$460
CD247 / CD3 Zeta Antibody - Anti-CD247 / CD3-Zeta antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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CD247 / CD3 Zeta Mouse anti-Human Monoclonal (4A12-F6) Antibody
Human
ELISA, IHC, IHC-P, IP, WB
Unconjugated
50 µg/$440
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CD247 / CD3 Zeta Hamster anti-Mouse Monoclonal (Biotin) (H146-968) Antibody
Mouse
ELISA, Flo, WB
Biotin Conjugated
100 µg/$339; 300 µg/$550
CD247 / CD3 Zeta Antibody - Immunofluorescence analysis of A549 cells.
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CD247 / CD3 Zeta Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
Select
CD247 / CD3 Zeta Hamster anti-Mouse Monoclonal (FITC) (H146-968) Antibody
Mouse
ELISA, Flo, WB
FITC Conjugated
100 µg/$358; 300 µg/$534
CD247 / CD3 Zeta Antibody - Western blot analysis of CD247 expression in Jurkat (A); NS-1 (B); PC12 (C) whole cell lysates.
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CD247 / CD3 Zeta Rabbit anti-Human Polyclonal (Internal) Antibody
Rabbit, Mouse, Rat, Pig, Human, Monkey
WB
Unconjugated
100 µl/$299
CD247 / CD3 Zeta Antibody - CD3z Antibody in Immunohistochemistry (IHC)
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CD247 / CD3 Zeta Mouse anti-Human Monoclonal (4B10) Antibody
Human
Flo, ICC, IF, IHC, WB
Unconjugated
100 µl/$541
CD247 / CD3 Zeta Antibody - Immunohistochemistry (IHC) analysis of paraffin-embedded human Thymus tissues with AEC staining using CD247 Monoclonal Antibody.
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CD247 / CD3 Zeta Mouse anti-Human Monoclonal Antibody
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
50 µg/$332; 100 µg/$404; 200 µg/$505
CD247 / CD3 Zeta Antibody - Immunohistochemistry of paraffin-embedded human lymph node tissue using CD247 Antibody at dilution of 1:100
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CD247 / CD3 Zeta Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$294; 100 µg/$360
CD247 / CD3 Zeta Antibody - 1:100 staining human Head and neck carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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CD247 / CD3 Zeta Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Select
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal Antibody
Human
ELISA, Flo, IHC, IP, WB
Unconjugated
100 µg/$331
Select
CD247 / CD3 Zeta Rabbit Recombinant Monoclonal Antibody
Flo, IHC, IHC-IF, IP, WB
Unconjugated
10 µl/$299; 100 µl/$548
CD247 / CD3 Zeta Antibody - Detection of phosphorylated CD3 zeta (EM-26; light blue) in immunological synapse formed between the lymph node naïve T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.
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CD247 / CD3 Zeta Mouse anti-Human Monoclonal (pTyr72) (EM-26) Antibody
Mouse, Human
Flo, ICC, WB
Unconjugated
0.1 mg/$302
Select
CD247 / CD3 Zeta Hamster anti-Mouse Monoclonal (H146-968) Antibody
Mouse
ELISA, Flo, WB
Unconjugated
250 µg/$433
Select
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal (80-160 aa) Antibody
Mouse, Rat, Human, Monkey
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
Select
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal (80-160 aa) Antibody
Mouse, Rat, Human, Monkey
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
CD247 / CD3 Zeta Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Select
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
CD247 / CD3 Zeta Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Select
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal (pTyr142) Antibody
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
CD247 / CD3 Zeta Antibody - Flow cytometric (FCM) analysis CD3 expressed in human peripheral blood cells using CD3 Monoclonal Antibody.
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CD247 / CD3 Zeta Mouse anti-Human Monoclonal Antibody
Human
ELISA, Flo
Unconjugated
50 µg/$332; 100 µg/$404; 200 µg/$505
Select
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal (aa2-31) (Biotin) Antibody
Human
ELISA, Flo, WB
Biotin Conjugated
200 µl/$952
Select
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal (aa2-31) (Azide-free) (HRP) Antibody
Human
ELISA, Flo, WB
HRP Conjugated, Azide-free
200 µl/$952
CD247 / CD3 Zeta Antibody - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.
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CD247 / CD3 Zeta Mouse anti-Human Monoclonal (pTyr153) (EM-17) Antibody
Mouse, Human
Flo, WB
Unconjugated
0.1 mg/$302
CD247 / CD3 Zeta Antibody - Cell lyzates of HEK293T/17 cells transiently transfected  with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54).    1: Wt mCD3zeta pervanadate non-stimulated  2: Wt mCD3zeta pervanadate  stimulated  3: mut. mCD3zeta Y/F2-6  4: mut. mCD3zeta Y/F1 and 3-6  5: mut. mCD3zeta Y/F1-2 and 4-6  6: mut. mCD3zeta Y/F1-3 and 5-6  7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native)  8: mut. mCD3zeta Y/F1-5
Select
CD247 / CD3 Zeta Mouse anti-Human Monoclonal (pTyr142) (EM-54) Antibody
Mouse, Human
Flo, WB
Unconjugated
0.1 mg/$302
CD247 / CD3 Zeta Antibody - Cell lyzates of HEK293T/17 cells transiently transfected  with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55).    1: Wt mCD3zeta pervanadate non-stimulated  2: Wt mCD3zeta pervanadate  stimulated  3: mut. mCD3zeta Y/F2-6  4: mut. mCD3zeta Y/F1 and 3-6  5: mut. mCD3zeta Y/F1-2 and 4-6 (thus only pY111 remains native)  6: mut. mCD3zeta Y/F1-3 and 5-6  7: mut. mCD3zeta Y/F1-4 and 6   8: mut. mCD3zeta Y/F1-5
Select
CD247 / CD3 Zeta Mouse anti-Human Monoclonal (pTyr111) (EM-55) Antibody
Mouse, Human
Flo, WB
Unconjugated
0.1 mg/$302
Select
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).