Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers

Customer Service: (800) 227-6666 / (650) 697-3600

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-C662908-0.1	0.1 mg (1 mg/ml)	$302

CD247 / CD3 Zeta Antibody - Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY142 mCD3zeta (clone EM-54). 1: Wt mCD3zeta pervanadate non-stimulated 2: Wt mCD3zeta pervanadate stimulated 3: mut. mCD3zeta Y/F2-6 4: mut. mCD3zeta Y/F1 and 3-6 5: mut. mCD3zeta Y/F1-2 and 4-6 6: mut. mCD3zeta Y/F1-3 and 5-6 7: mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native) 8: mut. mCD3zeta Y/F1-5

Monoclonal Mouse anti‑Human CD247 / CD3 Zeta Antibody (clone EM‑54, phospho‑Tyr142, WB) LS‑C662908

Name: Anti-CD247 / CD3 Zeta Antibody | Mouse anti-Human Monoclonal Flo,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662908-0.1
Price: 302.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

CD247 / CD3 Zeta Mouse anti-Human Monoclonal (pTyr142) (EM-54) Antibody

Application:

WB, Flo

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$302

LS-C662908-0.1

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CD247 / CD3 Zeta Mouse anti-Human Monoclonal (pTyr142) (EM-54) Antibody

Application:

WB, Flo

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

CD3 Zeta antibody LS-C662908 is an unconjugated mouse monoclonal antibody to CD3 Zeta (CD247) (pTyr142) from human. It is reactive with human and mouse. Validated for Flow and WB.

Target

Human CD247 / CD3 Zeta

Synonyms

See All CD247 / CD3 Zeta Antibodies

Host

Mouse

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Clone

EM-54

Conjugations

Unconjugated

Purification

Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.

Modifications

Unmodified

Epitope

pTyr142

Specificity

The mouse monoclonal antibody EM-54 recognizes phosphorylated tyrosine 142 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Applications