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Catalog Number Size Price
LS-C801498-100 100 µl (1 mg/ml) $379 
LS-C801498-200 200 µl (1 mg/ml) $421 
CD247 / CD3 Zeta Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CD247 / CD3 Zeta Antibody - Western blot analysis of CD3 ? (Phospho-Tyr142) expression in Jurkat cells lysate. The lane on the left is treated with the antigen-specific peptide.
CD247 / CD3 Zeta Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CD247 / CD3 Zeta Antibody - Western blot analysis of CD3 ? (Phospho-Tyr142) expression in Jurkat cells lysate. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human CD247 / CD3 Zeta Antibody (phospho‑Tyr142, IF, WB) LS‑C801498

Polyclonal Rabbit anti‑Human CD247 / CD3 Zeta Antibody (phospho‑Tyr142, IF, WB) LS‑C801498

Antibody:
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal (pTyr142) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801498-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD247 / CD3 Zeta Rabbit anti-Human Polyclonal (pTyr142) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CD3 Zeta antibody LS-C801498 is an unconjugated rabbit polyclonal antibody to CD3 Zeta (CD247) (pTyr142) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
Target
Human CD247 / CD3 Zeta
Synonyms
CD247 | CD3 Zeta | CD3H | CD3Z | CD247 antigen, zeta subunit | CD247 molecule | CD3zeta chain | T3Z | TCR zeta chain | TCRZ | T-cell receptor T3 zeta chain | CD247 antigen | CD3-ZETA | CD3Q
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human CD3 zeta (Phospho-Tyr142).
Epitope
pTyr142
Specificity
CD3 zeta (Phospho-Tyr142) Antibody detects endogenous levels of total CD3 zeta (Phospho-Tyr142).
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD247 / CD3 Zeta
P20963 NM_000734 NP_000725.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024