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APRT

adenine phosphoribosyltransferase

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: adenine phosphoribosyltransferase
Synonyms: APRT, AMP, AMP diphosphorylase, APRTD, AMP pyrophosphorylase, Transphosphoribosidase
Target Sequences: NM_000485 NP_000476.1 P07741

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Products
Antibodies (47)
Primary (47)
APRT (47)
Human (47)
Mouse (6)
Rat (8)
Chimpanzee (3)
Dog (3)
IHC (20)
IHC-Fr (1)
IHC-P (10)
WB (45)
Flo (3)
ELISA (14)
ICC (3)
IF (4)
IP (1)
Peptide-ELISA (1)
rabbit (46)
mouse (1)
Product Group
IHCPlus (2)
IgG (34)
polyclonal pc (47)
APC (1)
APC Conjugated (4)
Azide-free (2)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (4)
Preservative Free (1)
Unconjugated (22)
aa1-180 (14)
aa12-40 (7)
aa143-170 (7)
N-Terminus (4)
aa5-49 (2)
aa1-84 (1)
No (47)
APRT Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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APRT Rabbit anti-Human Polyclonal (aa12-40) Antibody
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400 µl/$393
APRT Antibody - Anti-APRT antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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APRT Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
APRT Antibody - Anti-APRT antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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APRT Rabbit anti-Human Polyclonal (N-Terminus) (HRP) Antibody
Dog, Rat, Chimpanzee, Human
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
APRT Antibody - Anti-APRT antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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APRT Rabbit anti-Human Polyclonal (N-Terminus) (Biotin) Antibody
Dog, Rat, Chimpanzee, Human
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
APRT Antibody - Anti-APRT antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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APRT Rabbit anti-Human Polyclonal (N-Terminus) (FITC) Antibody
Dog, Rat, Chimpanzee, Human
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
APRT Antibody - Immunofluorescent analysis of APRT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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APRT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ICC, IF, WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
APRT Antibody - Immunohistochemistry of paraffin-embedded human prostate using APRT Antibody at dilution of 1:100 (40x lens).
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APRT Rabbit anti-Human Polyclonal (aa1-180) Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
APRT Antibody - Western blot analysis of APRT using anti-APRT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APRT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APRT at approximately 20KD. The expected band size for APRT is at 20KD.
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APRT Rabbit anti-Human Polyclonal (aa5-49) Antibody
Human
Flo, ICC, IHC, IHC-Fr, WB
Unconjugated
10 µg/$318; 100 µg/$470
APRT Antibody - Human, Prostate: Formalin-Fixed Paraffin-Embedded (FFPE)
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APRT Rabbit anti-Human Polyclonal (aa143-170) Antibody
Human
IHC, IHC-P, WB
Unconjugated
200 µl/$460
APRT Antibody - IHC of paraffin-embedded DLD-1 xenograft using APRT antibody at 1:500 dilution.
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APRT Rabbit anti-Human Polyclonal (aa1-84) Antibody
Human
ICC, IHC, IHC-P, WB
Unconjugated
100 µl/$402
APRT Antibody - Immunofluorescence analysis of A549 cells.
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APRT Rabbit anti-Human Polyclonal (aa1-180) Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
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APRT Rabbit anti-Human Polyclonal (aa12-40) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
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APRT Rabbit anti-Human Polyclonal (aa12-40) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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APRT Rabbit anti-Human Polyclonal (aa12-40) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
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APRT Rabbit anti-Human Polyclonal (aa12-40) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
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APRT Rabbit anti-Human Polyclonal (aa12-40) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
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APRT Rabbit anti-Human Polyclonal (aa12-40) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
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APRT Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$331
APRT Antibody - Immunohistochemistry of paraffin-embedded human liver cancer using antibody at 1:100 dilution.
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APRT Rabbit anti-Human Polyclonal (aa1-180) Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$294; 100 µl/$360
APRT Antibody - 1:100 staining human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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APRT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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APRT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, WB
Unconjugated
50 µl/$356; 100 µl/$461
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APRT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
APRT Antibody - Western blot testing of human K562 cell lysate with APRT antibody at 0.5ug/ml. Predicted molecular weight ~20 kDa.
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APRT Rabbit anti-Human Polyclonal (aa5-49) Antibody
Human
Flo, WB
Unconjugated
100 µg/$575
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APRT Rabbit anti-Human Polyclonal (aa143-170) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
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APRT Rabbit anti-Human Polyclonal (aa1-180) Antibody
Human
IP, WB
Unconjugated
100 µl/$749
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).