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Antibodies > APRT > LS-C156439
 
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Catalog Number Size Price
LS-C156439-400 400 µl $393 
APRT Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
APRT Antibody - Fluorescent image of A549 cell stained with APRT Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.
APRT Antibody - APRT Antibody western blot of A2058 cell line lysates (35 ug/lane). The APRT antibody detected the APRT protein (arrow).
APRT Antibody - Flow cytometric of HepG2 cells using APRT Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
APRT Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
APRT Antibody - Fluorescent image of A549 cell stained with APRT Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.
APRT Antibody - APRT Antibody western blot of A2058 cell line lysates (35 ug/lane). The APRT antibody detected the APRT protein (arrow).
APRT Antibody - Flow cytometric of HepG2 cells using APRT Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human APRT Antibody (aa12‑40, IHC, IF, WB) LS‑C156439

Polyclonal Rabbit anti‑Human APRT Antibody (aa12‑40, IHC, IF, WB) LS‑C156439

Submit Review LSBio Guarantee
Antibody:
APRT Rabbit anti-Human Polyclonal (aa12-40) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C156439-400
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APRT Rabbit anti-Human Polyclonal (aa12-40) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
APRT antibody LS-C156439 is an unconjugated rabbit polyclonal antibody to human APRT (aa12-40). Validated for Flow, IF, IHC and WB.
Target
Human APRT
Synonyms
APRT | AMP | AMP diphosphorylase | APRTD | AMP pyrophosphorylase | Transphosphoribosidase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa12-40
Specificity
This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human APRT.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APRT
P07741 NM_000485 NP_000476.1

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Featured Products

APRT Antibody - Anti-APRT antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
APRT Antibody (N‑Terminus) LS‑B10419
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
APRT Antibody - Immunofluorescent analysis of APRT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
APRT Antibody LS‑C482448
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
APRT Antibody - Immunohistochemistry of paraffin-embedded mouse lung using APRT Antibody at dilution of 1:100 (40x lens).
APRT Antibody (aa1‑180) LS‑C748961
Species: Human, Mouse, Rat
Applications: IHC, Western blot
See all APRT Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
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