Target
LIF
Synonyms
LIF | DIA | Emfilermin | HILDA | Melanoma-derived LPL inhibitor | MLPLI | Leukemia inhibitory factor | CDF | D factor | Human interleukin in DA cells
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
This 180 amino acid protein has a predicted molecular mass of approximately 20 kD. The protein migrates at approximately 20 kD in DTT-reducing conditions and approximately 18 kD in non-reducing condition by SDS-PAGE. The predicted N-terminal amino acid is
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Mouse LIF inhibits proliferation of M-1 cells with an ED50 of 0.05 - 0.2 ng/mL.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
0.22 µm filtered protein solution in PBS, pH 7.4
Storage
Store unopened at 2°C to 8°C for up to 1 week, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee