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| Catalog Number | Size | Price |
|---|---|---|
| LS-G3928-10 | 10 µg | $309 |
Human LIF Protein (Recombinant) (aa23-202) - LS-G3928
Human LIF Protein (Recombinant) (aa23-202) - LS-G3928
Description:
LIF Protein LS-G3928 is a Recombinant Human LIF produced in E. coli aa 23-202. It is low in endotoxin; Less than 0.05 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
LIF Protein LS-G3928 is a Recombinant Human LIF produced in E. coli aa 23-202. It is low in endotoxin; Less than 0.05 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
LIF
Synonyms
LIF | DIA | Emfilermin | HILDA | Melanoma-derived LPL inhibitor | MLPLI | Leukemia inhibitory factor | CDF | D factor | Human interleukin in DA cells
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Region
aa 23-202
Predicted Molecular Weight
~46kDa (SDS-PAGE)
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.
Endotoxin
Less than 0.05 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with 100 µl sterile water.
Storage
Store lyophilized at -20°C for up to 6 months. Once reconstituted store at 4°C for immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
About LIF
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Images
Functional Assay
Figure: Human Leukemia Inhibitory Factor (LIF) (rec.) (AG-40B-0093) maintains pluripotency of mouse ES cells. Method: Mouse ES oct4 GFP cells were cultured for 3 days in the presence of the indicated concentrations of LIF and followed by flow cytometry analysis of the GFP expression (indicating the expression of Oct4, thus of pluripotency).
Functional Assay
Figure: Human Leukemia Inhibitory Factor (LIF) (rec.) (AG-40B-0093) maintains pluripotency of mouse ES cells. Method: Mouse ES oct4 GFP cells were cultured for 3 days in the presence of the indicated concentrations of LIF and followed by flow cytometry analysis of the GFP expression (indicating the expression of Oct4, thus of pluripotency).
Functional Assay
Figure: Human Leukemia Inhibitory Factor (LIF) (rec.) (AG-40B-0093) maintains pluripotency of mouse ES cells. Method: Mouse ES oct4 GFP cells were cultured for 3 days in the presence of the indicated concentrations of LIF and followed by flow cytometry analysis of the GFP expression (indicating the expression of Oct4, thus of pluripotency).
Functional Assay
Figure: Human Leukemia Inhibitory Factor (LIF) (rec.) (AG-40B-0093) maintains pluripotency of mouse ES cells. Method: Mouse ES oct4 GFP cells were cultured for 3 days in the presence of the indicated concentrations of LIF and followed by flow cytometry analysis of the GFP expression (indicating the expression of Oct4, thus of pluripotency).
Popular LIF Proteins
Source:
Human
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
