Target
FST / Follistatin
Synonyms
FST | Activin-binding protein | Follistatin isoform FST317 | Follistatin | Fs
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant mouse FST comprises 326 amino acids and has a predicted molecular mass of 36.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFST is approximately 70-80 kDa due to glycosylation.
Expression System
HEK 293 Cells
Purification
> 98 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 0.02-0.08 µg/mL in the presence of 10 ng/ml Recombinant Human Activin A.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee