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Catalog Number Size Price
LS-G81212-20 20 µg $215 
SHH / Sonic Hedgehog Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human SHH / Sonic Hedgehog Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G81212

Human SHH / Sonic Hedgehog Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G81212

Description:
SHH / Sonic Hedgehog Protein LS-G81212 is an Over-Expression Lysate Human SHH / Sonic Hedgehog with Myc-DDK (Flag) tag(s). For Research Use Only
Price
Catalog Number
$215
LS-G81212-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
SHH / Sonic Hedgehog Protein LS-G81212 is an Over-Expression Lysate Human SHH / Sonic Hedgehog with Myc-DDK (Flag) tag(s). For Research Use Only

Specifications

Type
Over-Expression Lysate Protein
Target
SHH / Sonic Hedgehog
Synonyms
SHH | HHG-1 | HLP3 | Sonic hedgehog | Sonic hedgehog homolog | MCOPCB5 | Sonic hedgehog protein | HHG1 | HPE3 | SMMCI | TPT | TPTPS
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Myc-DDK (Flag)
Predicted Molecular Weight
50.1 kDa
AA Sequence
MGEMLLLARCLLLVLVSSLLVCSGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLSPGDRVLAADDQGRLLYSDFLTFLDRDDGAKKVFYVIETREPRERLLLTAAHLLFVAPHNDSATGEPEASSGSGPPSGGALGPRALFASRVRPGQRVYVVAERDGDRRLLPAAVHSVTLSEEAAGAYAPLTAQGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDRGGDSGGGDRGGGGGRVALTAPGAADAPGAGATAGIHWYSQLLYQIGTWLLDSEALHPLGMAVKSS(CloningSiteandTags:TRTRPLEQKLISEEDLAANDILDYKDDDDKV)
Expression System
293T Cells
Source Species
Human
Purification
Unpurified
Usage Summary
Western blot: Spin down briefly and resuspend in 2X SDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT). Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Bio-Activity
Not Tested
Endotoxin
Not Tested
Presentation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Reconstitution
Reconstitute with SDS Sample Buffer
Storage
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About SHH / Sonic Hedgehog
Q15465 NM_000193 NP_000184.1

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Images

Western blot

SHH / Sonic Hedgehog Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

SHH / Sonic Hedgehog Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

SHH / Sonic Hedgehog Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

SHH / Sonic Hedgehog Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024