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| Catalog Number | Size | Price |
|---|---|---|
| LS-G375-5 | 5 µg | $268 |
| LS-G375-25 | 25 µg | $349 |
Mouse SHH / Sonic Hedgehog Protein (Recombinant) - LS-G375
Mouse SHH / Sonic Hedgehog Protein (Recombinant) - LS-G375
Description:
SHH / Sonic Hedgehog Protein LS-G375 is a Recombinant Mouse SHH / Sonic Hedgehog produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only
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(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
SHH / Sonic Hedgehog Protein LS-G375 is a Recombinant Mouse SHH / Sonic Hedgehog produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.1 ng/µg of protein (less than 1EU/µg). For Research Use Only
Specifications
Type
Recombinant Protein
Target
SHH / Sonic Hedgehog
Synonyms
SHH | HHG-1 | HLP3 | Sonic hedgehog | Sonic hedgehog homolog | MCOPCB5 | Sonic hedgehog protein | HHG1 | HPE3 | SMMCI | TPT | TPTPS
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Predicted Molecular Weight
20.0 kDa
AA Sequence
IVIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 90% by SDS-PAGE and HPLC
Bio-Activity
Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.5-1.0 ug/ml.
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from sodium phosphate, pH 5.5, 0.15 M sodium chloride, 0.5 mM DTT
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
