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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16214-0.05 | 0.05 ml | $460 |
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PathPlus™ Monoclonal Mouse anti‑Human PDPN / Podoplanin Antibody (Concentrated, IHC) LS‑B16214
PathPlus™ Monoclonal Mouse anti‑Human PDPN / Podoplanin Antibody (Concentrated, IHC) LS‑B16214
Note: This antibody replaces LS-C757388
Antibody:
PDPN / Podoplanin Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PDPN / Podoplanin Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Specifications
Description
PDPN (podoplanin) is mucin-type membrane glycoprotein with expression in a diverse range of tissues and cell types including lung alveolar cells, kidney podocytes, lymphatic endothelial cells, and neural tissues. PDPN is a required protein in the development of the lungs, lymphatic system and the heart, and it separately may play a role in immune response to fungal infections. In immunohistochemistry, PDPN has cytoplasmic and membranous staining in the lymphatic endothelium, breast myoepithelium, podocytes, dendritic cells and mesothelium. It is a sensitive and specific marker for the lymphatic endothelium, and it is used as an endothelial cell marker in determining lymphatic invasion in tumors.
References: Clin Inv. 118 (11): 3725–37, PMID: 18924607; Frontiers in Immunology. 2012. 3: 283. doi:10.3389/fimmu.2012.00283, PMID: 22988448
Target
Human PDPN / Podoplanin
Synonyms
PDPN | AGGRUS | HT1alpha-1 | Glycoprotein 36 | Glycoprotein, 36-KD | gp36 | gp38 | OTS8 | PA2.26 | PA2.26 antigen | Podoplanin | T1A | gp40 | HT1A-1 | HT1alpha-2 | p38 | T1-alpha | T1A-2
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Concentrated.
Also available Ready-to-Use.
Immunogen
Recombinant Human Podoplanin
Applications
- IHC - Paraffin (1:100)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PDPN / Podoplanin
Validation
Human Breast, myoepithelial cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast, myoepithelial cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ PDPN / Podoplanin Antibody (Concentrated) for IHC, ELISA LS-B16214 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, IHC - Frozen
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human
Applications:
Immunofluorescence, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
