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Catalog Number Size Price
LS-B16444-100 100 µg (0.5 mg/ml) $485 
HSPD1 / HSP60 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - HSPD1 / HSP60 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
HSPD1 / HSP60 Antibody - HSPD1 / HSP60 antibody (0.1µg/ml) staining of Rat Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
HSPD1 / HSP60 Antibody - HSPD1 / HSP60 antibody (0.03µg/ml) staining of Human Heart (A), Kidney (B) and Liver (C) lysate and in Pig Heart (D), Kidney (E) and Liver (F) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
HSPD1 / HSP60 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPD1 / HSP60 Antibody - HSPD1 / HSP60 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
HSPD1 / HSP60 Antibody - HSPD1 / HSP60 antibody (0.1µg/ml) staining of Rat Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
HSPD1 / HSP60 Antibody - HSPD1 / HSP60 antibody (0.03µg/ml) staining of Human Heart (A), Kidney (B) and Liver (C) lysate and in Pig Heart (D), Kidney (E) and Liver (F) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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PathPlus™ Polyclonal Goat anti‑Human HSPD1 / HSP60 Antibody (IHC, IF, WB) LS‑B16444

PathPlus™ Polyclonal Goat anti‑Human HSPD1 / HSP60 Antibody (IHC, IF, WB) LS‑B16444

Note: This antibody replaces LS-C502369
Antibody:
HSPD1 / HSP60 Goat anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Rat, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B16444-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPD1 / HSP60 Goat anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Rat, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
HSPD1 heat shock protein family D member 1 (HSP60) is a member of the mitochondrial chaperonin family that is required for the folding and assembly of proteins in the mitochondria. HSPD1 is also an interferon regulatory factor 3-interacting protein whose overexpression enhances activation of the IFN-beta promoter, contributing to the induction of the IFN-beta signaling pathway. Mutations in this gene cause some forms of hereditary spastic paraplegia. It is expressed in the adrenal, kidney, skeletal muscle, and many other tissues. References: Front Mol biosci 2016 3:49; PlosOne 2014 9(12):e114874; Science Direct 2002 70(5):1328
Target
Human HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Goat
Reactivity
Human, Rat, Pig (tested or 100% immunogen sequence identity)
Predicted
Dog, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-NLEDVQPHDLGK, from the internal region of the protein sequence according to NP_002147.2.
Applications
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.01 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPD1 / HSP60
P10809 NM_002156 NP_002147.2

Validation

HSPD1 / HSP60 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPD1 / HSP60 Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPD1 / HSP60 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ HSPD1 / HSP60 Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B16444 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/30/2024