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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2946-100 | 100 µl (1 mg/ml) | $460 |
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PathPlus™ Monoclonal Mouse anti‑Human HSPD1 / HSP60 Antibody (clone 2E4, aa1‑573, IHC, WB) LS‑B2946
PathPlus™ Monoclonal Mouse anti‑Human HSPD1 / HSP60 Antibody (clone 2E4, aa1‑573, IHC, WB) LS‑B2946
Note: This antibody replaces LS-C93750
Antibody:
HSPD1 / HSP60 Mouse anti-Human Monoclonal (aa1-573) (2E4) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
HSPD1 / HSP60 Mouse anti-Human Monoclonal (aa1-573) (2E4) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
HSPD1 heat shock protein family D member 1 (HSP60) is a member of the mitochondrial chaperonin family that is required for the folding and assembly of proteins in the mitochondria. HSPD1 is also an interferon regulatory factor 3-interacting protein whose overexpression enhances activation of the IFN-beta promoter, contributing to the induction of the IFN-beta signaling pathway. Mutations in this gene cause some forms of hereditary spastic paraplegia. It is expressed in the adrenal, kidney, skeletal muscle, and many other tissues.
References: Front Mol biosci 2016 3:49; PlosOne 2014 9(12):e114874; Science Direct 2002 70(5):1328
Target
Human HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
2E4
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human HSP60 (1-573aa) purified from E. coli.
Epitope
aa1-573
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000 - 1:3000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2946 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2946 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSPD1 / HSP60
Validation
Anti-HSPD1 antibody IHC of human placenta, trophoblasts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human placenta, trophoblasts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HSPD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
PathPlus™ HSPD1 / HSP60 Antibody (aa1-573, clone 2E4) for IHC, WB/Western, ELISA LS-B2946 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken, Xenopus, Drosophila
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken, C. elegans, Plant
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Xenopus, Fish, Drosophila, Bacteria, Beluga
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Rat, Pig
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/15/2024
Date Requested: 11/15/2024
