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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2031-50 | 50 µg (1 mg/ml) | $460 |
1 of 2
2 of 2
PathPlus™ Polyclonal Rabbit anti‑Human HAVCR1 / KIM‑1 Antibody (aa200‑300, IHC, WB) LS‑B2031
PathPlus™ Polyclonal Rabbit anti‑Human HAVCR1 / KIM‑1 Antibody (aa200‑300, IHC, WB) LS‑B2031
Note: This antibody replaces LS-C685
Antibody:
HAVCR1 / KIM-1 Rabbit anti-Human Polyclonal (aa200-300) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
HAVCR1 / KIM-1 Rabbit anti-Human Polyclonal (aa200-300) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
HAVCR1 (KIM-1, TIM-1, CD365) is a member of the TIM family, with functional roles in asthma, transplant tolerance, allergic response and regulating immune cell activities. HAVCR1 is located on the surface of Type 2 helper T cells cells and is capable of activating T-cells and blocking peripheral tolerance. It is highly upregulated in injured kidneys. Additionally, it is a receptor for Hepatitis A virus, Ebola and Dengue virus. It is potentially a useful prognostic marker for renal cell, gastric and other cancers, where upregulation is correlated with progression and is linked to angiogenesis and proliferation during tumorigenesis. In immunohistochemistry of normal tissue, HAVCR1 has cytoplasmic and membranous positivity in glandular epithelia of various tissues, with prominent expression in the kidney and gastrointestinal tract.
References: Cancer Res. 2014 Mar 1;74(5):1416-28, PMID: 24390735; PLoS One. 2018 Nov 2;13(11):e0206423, PMID: 30388143; J Virol. 2018 Apr 13;92(9). pii: e02065-17, PMID: 29437974; Arch Pathol Lab Med. 2014 Dec;138(12):1643-65, PMID: 25427043
Target
Human HAVCR1 / KIM-1
Synonyms
HAVCR1 | HAVCR-1 | KIM-1 | Kidney injury molecule 1 | KIM1 | T-cell membrane protein 1 | TIM1 | TIMD1 | TIM-1 | HAVCR | TIMD-1 | CD365
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A synthetic peptide of human HAVCR1/KIM-1 protein was used as the immunogen. This sequence detects both isoform a and isoform CRA_a (accessions NP_036338 and EAW61615.1 respectively). Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey (93%); Dog (80%).
Epitope
aa200-300
Specificity
The peptide used to produce this antibody is 100% homologous in human, 93% in vervet/green monkey and 80% in chimp. Furthermore, the peptide sequence is conserved across HAVCR1 isoform a (364 amino acids, accession NP_036338) and HAVCR1 isoform CRA_a (194 amino acids, accession EAW61615.1). As isoforms of different molecular weights have been described for this protein, the molecular weight observed in western blots may vary depending on the forms present.
Applications
- IHC
- IHC - Paraffin (2.5 - 5 µg/ml)
- Western blot (1 - 3 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2031 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2031 was determined to be 2.5-5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HAVCR1 / KIM-1
Validation
Anti-TIM-1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TIM-1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ HAVCR1 / KIM-1 Antibody (aa200-300) for IHC, WB/Western LS-B2031 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Urinary KIM-1 Correlates with the Subclinical Sequelae of Tubular Damage Persisting after the Apparent Functional Recovery from Intrinsic Acute Kidney Injury. Cristina Cuesta, Isabel Fuentes-Calvo, Sandra M Sancho-Martinez, Floris A Valentijn, Annette Düwel, Omar A Hidalgo-Thomas, Consuelo Agüeros-Blanco, Adalberto Benito-Hernández, María A Ramos-Barron, Carlos Gómez-Alamillo, Manuel Arias, Tri Q Nguyen, Roel Goldschmeding, Carlos Martínez-Salgado, Francisco J López-Hernández. Biomedicines. 2022 May;10:
Customer Reviews (0)
Featured Products
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
