PathPlus™ Monoclonal Mouse anti‑Human EP300 / p300 Antibody (clone RW128, aa1572‑2371, IHC, IF, WB) LS‑B215
PathPlus™ Monoclonal Mouse anti‑Human EP300 / p300 Antibody (clone RW128, aa1572‑2371, IHC, IF, WB) LS‑B215
Note: This antibody replaces LS-C2747, LS-C91230, LS-C91229, LS-C2745
Antibody:
EP300 / p300 Mouse anti-Human Monoclonal (aa1572-2371) (RW128) Antibody
Application:
IHC-P, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Mustelid, Primate
Format:
Unconjugated, Unmodified
Overview
Antibody:
EP300 / p300 Mouse anti-Human Monoclonal (aa1572-2371) (RW128) Antibody
Application:
IHC-P, IF, WB, IP
Reactivity:
Human, Mouse, Rat, Mustelid, Primate
Format:
Unconjugated, Unmodified
Specifications
Description
EP300 is a histone acetyltransferase that regulates transcription via chromatin remodeling. It acetylates all four core histones in nucleosomes. EP300 mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. It also plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. EP300 can also promote cardiac myocyte enlargement. In infectious disease, it binds to and may be involved in the transforming capacity of the adenovirus E1A protein, and in case of HIV-1 infection, it is recruited by the viral protein Tat. It regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. In immunohistochemistry, EP300 has nuclear and cytoplasmic positivity in a majority of tissues throughout the body.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804;
Target
Human EP300 / p300
Synonyms
EP300 | E1A binding protein p300 | Histone acetyltransferase p300 | KAT3B | p300 HAT | E1A-associated protein p300 | E1A-binding protein, 300kD | p300 | RSTS2
Reactivity
Human, Mouse, Rat, Mustelid, Primate
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Fusion protein containing residues 1572-2371 of human p300.
Specificity
P300 protein. This recognizes residues 1868-1921.
Applications
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence
- Western blot (1:250 - 1:500)
- Immunoprecipitation
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B215 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B215 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
LSBio Ratings
Laboratory Validation Score (5)
Featured Products
Species:
Human
Applications:
IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ChIP-sequencing
Species:
Human, Mouse
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/12/2024