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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3859-50 | 50 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human WNT5A Antibody (clone 6F2, IHC, IF, WB) LS‑B3859
IHC‑plus™ Monoclonal Mouse anti‑Human WNT5A Antibody (clone 6F2, IHC, IF, WB) LS‑B3859
Note: This antibody replaces LS-C108740, LS-C171455
Antibody:
WNT5A Mouse anti-Human Monoclonal (6F2) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
WNT5A Mouse anti-Human Monoclonal (6F2) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
WNT5A antibody LS-B3859 is an unconjugated mouse monoclonal antibody to human WNT5A. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human WNT5A
Synonyms
WNT5A | HWNT5A | Protein Wnt-5a | WNT-5A protein
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
6F2
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of WNT5A expressed in E. Coli.\
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200 - 1:400)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3859 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3859 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About WNT5A
Validation
Anti-WNT5A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-WNT5A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-WNT5A antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-WNT5A antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-WNT5A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-WNT5A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ WNT5A Antibody (clone 6F2) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3859 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Optimization, validation, and identification of two reliable antibodies for immunodetection of WNT5A. Prgomet Z, Andersson T, Lindberg P. Biotechnic & histochemistry : official publication of the Biological Stain Commission. 2017 92:46-58. (IHC; Human)
Differential Expression and Roles of Secreted Frizzled-Related Protein 5 and the Wingless Homolog Wnt5a in Periodontitis. Maekawa T, Kulwattanaporn P, Hosur K, Domon H, Oda M, Terao Y, Maeda T, Hajishengallis G. Journal of dental research. 2017 May;96:571-577.
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bovine
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, Western blot, Flow Cytometry, ELISA
![WNT5A Antibody - Immunoperoxidase of monoclonal antibody to WNT5A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/wnt5a-antibody-clone-3a4-ls-c133974/53453_1478301.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024
Date Requested: 11/24/2024
