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Antibodies > Vitamin D Receptor / VDR > LS-C801019
 
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Catalog Number Size Price
LS-C801019-100 100 µl (1 mg/ml) $379 
LS-C801019-200 200 µl (1 mg/ml) $421 
Vitamin D Receptor / VDR Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Vitamin D Receptor / VDR Antibody - Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
Vitamin D Receptor / VDR Antibody - Western blot analysis of Vitamin D Receptor phosphorylation expression in heatshock treated HT29 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
Vitamin D Receptor / VDR Antibody - Western blot analysis of Phospho-Vitamin D Receptor (Ser208) antibody expression in heatshock treated HT29 cells lysates. The lane on the right is treated with the antigen-specific peptide.
Vitamin D Receptor / VDR Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Vitamin D Receptor / VDR Antibody - Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
Vitamin D Receptor / VDR Antibody - Western blot analysis of Vitamin D Receptor phosphorylation expression in heatshock treated HT29 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
Vitamin D Receptor / VDR Antibody - Western blot analysis of Phospho-Vitamin D Receptor (Ser208) antibody expression in heatshock treated HT29 cells lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human Vitamin D Receptor / VDR Antibody (phospho‑Ser208, IF, WB) LS‑C801019

Polyclonal Rabbit anti‑Human Vitamin D Receptor / VDR Antibody (phospho‑Ser208, IF, WB) LS‑C801019

Submit Review LSBio Guarantee
Antibody:
Vitamin D Receptor / VDR Rabbit anti-Human Polyclonal (pSer208) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801019-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Vitamin D Receptor / VDR Rabbit anti-Human Polyclonal (pSer208) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
VDR antibody LS-C801019 is an unconjugated rabbit polyclonal antibody to human VDR (Vitamin D Receptor) (pSer208). Validated for IF, Peptide-ELISA and WB.
Target
Human Vitamin D Receptor / VDR
Synonyms
VDR | NR1I1 | Vitamin D3 receptor | Vitamin D receptor
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Vitamin D Receptor around the phosphorylation site of Serine 208.
Epitope
pSer208
Specificity
Phospho-Vitamin D Receptor (Ser208) Antibody detects endogenous levels of Vitamin D Receptor only when phosphorylated at Serine 208.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Vitamin D Receptor / VDR
P11473 NM_000376 NP_000367.1

Publications (0)

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Vitamin D Receptor / VDR Antibody - Immunofluorescence analysis of A549 cells, using Vitamin D Receptor (Phospho-Ser208) Antibody. The picture on the right is blocked with the phospho peptide.
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See all Vitamin D Receptor / VDR Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
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