Chicken Vitamin D Receptor / VDR

Rat

Chicken, Human, Mouse, Rat, Hamster, Pig, Frog (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

Vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear. Epitope: 89-105.

aa89-105

Recognizes both the occupied and unoccupied forms of VDR. Does not cross-react with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species cross-reactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (1 - 2 µg/ml)

Immunohistochemistry: LS-B2577 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2577 was determined to be 10 ug/ml. Positive control: LNCaP and HeLa cells.

PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P11473 NCBI: NM_000376 NP_000367.1