Human UCHL1 / PGP9.5

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Recombinant Monoclonal

rUCHL1/775

Unconjugated

Protein A/G purified

Unmodified. Also available Azide-free, Carrier-free.

Recombinant full-length human UCHL1 protein. Antigen Molecular Weight: 20-30 kDa

This MAb reacts with a protein of 20-30 kDa , identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

• IHC - Paraffin (1 - 2 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (1 - 2 µg/ml)
• Flow Cytometry (1 - 2 µg/10E6 cells)

Western Blotting (1-2 ug/ml) - Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS, 0.05% BSA, 0.05% Sodium Azide

Store at 2°C to 8°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09936 NCBI: NM_004181 NP_004172.2