Human UCHL1 / PGP9.5

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

31A3

Unconjugated

Protein G purified

Unmodified

Native, from brain

Recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubiquitin pathway. Stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the DNES. Clones 31A3 and each recognize a different epitope towards the N-terminus of the protein. Also recognizes PGP9.5 in other species, including rat and rabbit but evidence suggests it does not bind to PGP9.5 in guinea pigs. Has been used successfully as a capture reagent with clone 13C4 as a detection reagent in a sandwich ELISA to evaluate contamination of processed meat samples with neuronal tissue.

• IHC
• IHC - Paraffin (1:100 - 1:400)
• Western blot
• ELISA (1:500 - 1:2000)

For IHC-P specimens which have not been fixed in acetic acid/alcohol prior to embedding will require pretreatment using the microwave-citrate buffer method.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09936 NCBI: NM_004181 NP_004172.2