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| Catalog Number | Size | Price |
|---|---|---|
| LS-B489-50 | 50 µl (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human UCHL1 / PGP9.5 Antibody (aa100‑200, IHC, WB) LS‑B489
IHC‑plus™ Polyclonal Rabbit anti‑Human UCHL1 / PGP9.5 Antibody (aa100‑200, IHC, WB) LS‑B489
Note: This antibody replaces LS-C2926
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
PGP9.5 antibody LS-B489 is an unconjugated rabbit polyclonal antibody to human PGP9.5 (UCHL1) (aa100-200). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human UCHL1 / PGP9.5
Synonyms
UCHL1 | Ubiquitin C-terminal hydrolase | PARK5 | PGP9.5 | PGP95 | Ubiquitin thioesterase L1 | Neuron cytoplasmic protein 9.5 | PGP 9.5 | Uch-L1 | Ubiquitin carboxyl-terminal esterase L1 | HEL-117 | NDGOA | HEL-S-53 | SPG79
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide made to an internal portion of the human UCHL1 protein sequence (between residues 100-200). UniProt P09936. Percent identity by BLAST analysis: Primate, Guinea (100%); Rat (99%); Bovine (92%).
Epitope
aa100-200
Specificity
Rabbit Polyclonal to PGP9.5 - Peripheral Nerve Fiber marker.
Applications
- IHC
- IHC - Paraffin (2.5 - 5 µg/ml)
- Western blot (0.5 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B489 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B489 was determined to be 2.5-5 ug/ml.
Presentation
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About UCHL1 / PGP9.5
Validation
Anti-UCHL1 / PGP9.5 antibody IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-UCHL1 / PGP9.5 antibody IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ UCHL1 / PGP9.5 Antibody (aa100-200) for IHC, WB/Western LS-B489 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals. Lea RG, Amezaga MR, Loup B, Mandon-Pépin B, Stefansdottir A, Filis P, Kyle C, Zhang Z, Allen C, Purdie L, Jouneau L, Cotinot C, Rhind SM, Sinclair KD, Fowler PA. Scientific reports. 2016 6:22279. (WB; Human)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
