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Vector Laboratories, Inc.
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Customer Service: (800) 227-6666 / (650) 697-3600


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Catalog Number Size Price
LS-C662001-10 10 µg $318 
LS-C662001-100 100 µg $470 
TRPC6 Antibody - Western blot analysis of TRPC6 using anti-TRPC6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate,Lane 2: mouse lung tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD.

Polyclonal Rabbit anti‑Human TRPC6 Antibody (WB) LS‑C662001

Polyclonal Rabbit anti‑Human TRPC6 Antibody (WB) LS‑C662001

Antibody:
TRPC6 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662001-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TRPC6 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TRPC6 antibody LS-C662001 is an unconjugated rabbit polyclonal antibody to human TRPC6. Validated for WB.
Target
Human TRPC6
Synonyms
TRPC6 | Transient receptor protein 6 | Trrp6 | TRP-6 | TRP6 | FSGS2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human TRPC6 recombinant protein (Position: K771-E875). Human TRPC6 shares 82.9% amino acid (aa) sequence identity with mouse TRPC6.
Specificity
Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRPC6
Q9Y210 NM_004621 NP_004612.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024