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Antibodies > TRPC6 > LS-C312900
 
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Catalog Number Size Price
LS-C312900-10 10 µg $318 
LS-C312900-100 100 µg (0.5 mg/ml) $470 
TRPC6 Antibody - TRPC6 antibody. IHC(P): Rat Brain Tissue.
TRPC6 Antibody - TRPC6 antibody. IHC(P): Human Intestinal Cancer Tissue.
TRPC6 Antibody - Western blot analysis of TRPC6 using anti-TRPC6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung Tissue Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD.
TRPC6 Antibody - Western blot analysis of TRPC6 using anti-TRPC6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Lung Tissue Lysate Lane 2: 293T Cell Lysate Lane 3: 293T Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106-120KD. The expected band size for TRPC6 is at 106KD.
TRPC6 Antibody - WB of TRPC6 antibody. Lane 1: Rat Lung Tissue Lysate. Lane 2: 293T Cell Lysate. Lane 3: 293T Cell Lysate..
TRPC6 Antibody - TRPC6 antibody. IHC(P): Rat Brain Tissue.
TRPC6 Antibody - TRPC6 antibody. IHC(P): Human Intestinal Cancer Tissue.
TRPC6 Antibody - Western blot analysis of TRPC6 using anti-TRPC6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung Tissue Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD.
TRPC6 Antibody - Western blot analysis of TRPC6 using anti-TRPC6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Lung Tissue Lysate Lane 2: 293T Cell Lysate Lane 3: 293T Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106-120KD. The expected band size for TRPC6 is at 106KD.
TRPC6 Antibody - WB of TRPC6 antibody. Lane 1: Rat Lung Tissue Lysate. Lane 2: 293T Cell Lysate. Lane 3: 293T Cell Lysate..
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Polyclonal Rabbit anti‑Human TRPC6 Antibody (aa249‑265, IHC, WB) LS‑C312900

Polyclonal Rabbit anti‑Human TRPC6 Antibody (aa249‑265, IHC, WB) LS‑C312900

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Note: This antibody replaces LS-C389391
Antibody:
TRPC6 Rabbit anti-Human Polyclonal (aa249-265) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312900-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TRPC6 Rabbit anti-Human Polyclonal (aa249-265) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TRPC6 antibody LS-C312900 is an unconjugated rabbit polyclonal antibody to TRPC6 (aa249-265) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human TRPC6
Synonyms
TRPC6 | Transient receptor protein 6 | Trrp6 | TRP-6 | TRP6 | FSGS2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TRPC6(249-265 aa HDYFCKCNDCNQKQKHD), different from the related rat and mouse sequences by three amino acids.
Epitope
aa249-265
Specificity
Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRPC6
Q9Y210 NM_004621 NP_004612.2

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TRPC6 ELISA Kit
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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