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Catalog Number Size Price
LS-C796864-100 100 µg $539 
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody.  heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-TOP2A mouse monoclonal antibody.  heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using anti-TOP2A clone UMAB145 mouse monoclonal antibody at 1:333 with Polink2 Broad HRP DAB detection kit  heat-induced epitope retrieval with Accel buffer. (B22C, pH8.5)
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody.  heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
TOP2A / Topoisomerase II Alpha Antibody - Immunofluorescent staining of HeLa cells using anti-TOP2A mouse monoclonal antibody  green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin. (red), and nuclear with DAPI. (blue).
TOP2A / Topoisomerase II Alpha Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TOP2A mouse monoclonal antibody.  heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-TOP2A mouse monoclonal antibody.  heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using anti-TOP2A clone UMAB145 mouse monoclonal antibody at 1:333 with Polink2 Broad HRP DAB detection kit  heat-induced epitope retrieval with Accel buffer. (B22C, pH8.5)
TOP2A / Topoisomerase II Alpha Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOP2A mouse monoclonal antibody.  heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
TOP2A / Topoisomerase II Alpha Antibody - Immunofluorescent staining of HeLa cells using anti-TOP2A mouse monoclonal antibody  green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin. (red), and nuclear with DAPI. (blue).
TOP2A / Topoisomerase II Alpha Antibody - Western blot of cell lysates. (35ug) from 9 different cell lines. (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
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Monoclonal Mouse anti‑Human TOP2A / Topoisomerase II Alpha Antibody (clone UMAB145, Carrier‑free, aa1100‑1531, IHC, IF, WB) LS‑C796864

Monoclonal Mouse anti‑Human TOP2A / Topoisomerase II Alpha Antibody (clone UMAB145, Carrier‑free, aa1100‑1531, IHC, IF, WB) LS‑C796864

Antibody:
TOP2A / Topoisomerase II Alpha Mouse anti-Human Monoclonal (aa1100-1531) (Carrier-free) (UMAB145) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$539
LS-C796864-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TOP2A / Topoisomerase II Alpha Mouse anti-Human Monoclonal (aa1100-1531) (Carrier-free) (UMAB145) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
Topoisomerase II Alpha antibody LS-C796864 is an unconjugated mouse monoclonal antibody to human Topoisomerase II Alpha (TOP2A) (aa1100-1531). Validated for IF, IHC and WB.
Target
Human TOP2A / Topoisomerase II Alpha
Synonyms
TOP2A | DNA topoisomerase II, 170 kD | TOP2 | DNA gyrase | DNA topoisomerase 2-alpha | TP2A | Topoisomerase IIa
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
UMAB145
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Human recombinant protein fragment corresponding to amino acids 1100-1531 of human TOP2A(NP_001058) produced in E.coli.
Epitope
aa1100-1531
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:200)
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TOP2A / Topoisomerase II Alpha
P11388 NM_001067 NP_001058.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/22/2024